Functional characterization of the halogenase SpmH and discovery of new deschloro-tryptophan dimers
Literature Information
Zhiwen Liu, Liang Ma, Liping Zhang, Wenjun Zhang, Yiguang Zhu, Yuchan Chen, Weimin Zhang, Changsheng Zhang
The halogenase gene spmH was putatively involved in the biosynthesis of spiroindimicins/indimicins (SPMs/IDMs), a group of chlorinated tryptophan dimers (TDs) from deep-sea-derived Streptomyces sp. SCSIO 03032. Inactivation of spmH led to six deschloro-analogues of TDs, including four new compounds SPMs G (1) and H (2), and IDMs F (3) and G (4). The structures and absolute configurations of 1–4 were unambiguously determined by the combination of extensive spectroscopic analysis, single-crystal X-ray diffraction and quantum chemical ECD calculations. Compounds 1 and 2 exhibited moderate cytotoxic activities against four cancer cell lines. Additionally, SpmH was biochemically characterized in vitro as an L-tryptophan 5-halogenase.
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Organic & Biomolecular Chemistry

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