Raman spectroscopic study of Ni2+–DNA interactions in aqueous systems

Literature Information

Publication Date 2002-08-30
DOI 10.1039/B202346F
Impact Factor 3.676
Authors

Janusz Stangret, Rodrigue Savoie


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Abstract

The interactions of Ni2+ ion with calf thymus DNA has been studied in water at metal/DNA phosphate mole ratios ranging from 0.0125 to 6 using Raman spectroscopy. The weighted difference spectra method has been applied to analyze the data. It has been established that Ni2+ ion coordinates mainly at N7 centers of guanine (G) and adenine (A) of DNA at GG, AA and GA sequence places. At the same time the Ni2+ shows outer-sphere interaction, via water molecules, with phosphate groups of DNA.

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Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics
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