5-Nitrofluorescein(CAS: 3326-35-0)

5-Nitrofluorescein (CAS: 3326-35-0) is a water-soluble fluorescent dye. It crystallizes in the form of colorless needles or prisms. The molecular weight is 346.11 g/mol. It is soluble in water and methanol, but less soluble in ethanol. It exhibits high fluorescence intensity and good chemical stability under normal conditions. It is less reactive towards common organic reagents but can decompose under strong heat or light exposure.

5-Nitrofluorescein (CAS: 3326-35-0) is synthesized by the nitration of fluorescein. A mixture of fluorescein and a nitration reagent (such as nitric acid) is heated under controlled conditions. The product is then purified by recrystallization from a suitable solvent. Common catalysts include sulfuric acid, and the yield is typically around 70-80%. This process requires careful control of reaction conditions to ensure selectivity and high purity.

5-Nitrofluorescein (CAS: 3326-35-0) is widely used in various industries. It is a key reagent in analytical chemistry for fluorescence detection and quantification. It is also used in pharmaceuticals for marker probes, in polymers for fluorescent labeling, in sensors for detecting specific analytes, and in semiconductors for quality control during production processes. Its applications span from research laboratories to industrial settings.

When handling 5-Nitrofluorescein (CAS: 3326-35-0), it is important to use appropriate personal protective equipment (PPE) such as gloves, safety goggles, and a lab coat. Work should be conducted in a well-ventilated area or fume hood to minimize inhalation risks. Spills should be cleaned up immediately using suitable absorbent materials and disposed of according to local regulations. Always consult the Safety Data Sheet (SDS) for detailed guidelines on handling this compound safely.