TMB dihydrochloride(CAS: 64285-73-0)

TMB dihydrochloride, with the CAS number 64285-73-0, is a chemical compound commonly used as a chromogenic substrate in biochemical assays. It has the molecular formula C10H13N3O2·2HCl and is known for its stability in aqueous solutions. This compound is typically yellow in color and has a molecular weight of approximately 273.7 g/mol. It is widely recognized for its role in enzyme-linked immunosorbent assays (ELISA) and other diagnostic applications due to its strong absorption properties in the visible spectrum.

TMB dihydrochloride is primarily used in enzyme-linked immunosorbent assays (ELISA) as a chromogenic substrate for horseradish peroxidase (HRP). It is also applied in immunohistochemistry, immunoassays, and other biochemical research fields. Its sensitivity and ease of use make it a popular choice for detecting antigens and antibodies. Additionally, it finds applications in environmental testing and industrial quality control due to its reactivity and color development properties.

TMB dihydrochloride is generally considered safe when handled properly, but it should be treated with standard laboratory precautions. It may cause mild irritation to the skin and eyes, and inhalation of its vapors should be avoided. Safety data sheets (SDS) recommend using gloves, goggles, and a fume hood during handling. It is not classified as highly toxic, but proper disposal and adherence to safety guidelines are essential to ensure safe use in research and industrial settings.

TMB dihydrochloride should be stored in a cool, dry place away from direct light. It is typically kept in airtight containers at a temperature range of 2-8°C to maintain stability and prevent degradation. Proper storage conditions are crucial to preserve its chemical integrity and ensure consistent performance in assays. Avoid exposure to moisture and ensure the container is sealed tightly to maintain quality over time.