Tetramethylrhodamine-5-isothiocyanate(CAS: 80724-19-2)

Tetramethylrhodamine-5-isothiocyanate (CAS: 80724-19-2) is a yellow crystalline solid with a molecular weight of approximately 378.42 g/mol. It has poor water solubility but is soluble in organic solvents such as DMF, DMSO, and acetonitrile. It exhibits good fluorescence properties and is reactive towards amino groups, making it useful for conjugation with proteins and other biomolecules.

Tetramethylrhodamine-5-isothiocyanate is typically synthesized by reacting tetramethylrhodamine with carbonic anhydride. The reaction is carried out in an inert atmosphere to prevent decomposition. The yield is around 90%, and the product is isolated by column chromatography. No specific catalysts are required for this synthesis.

Tetramethylrhodamine-5-isothiocyanate (CAS: 80724-19-2) is widely used in the pharmaceutical industry for labeling of biomolecules, in polymer synthesis as a fluorescent marker, in sensor technology for detecting specific analytes, and in semiconductor applications for quality control and doping analysis.

When handling Tetramethylrhodamine-5-isothiocyanate (CAS: 80724-19-2), always wear appropriate personal protective equipment (PPE), including gloves, goggles, and lab coat. This compound should be handled in a well-ventilated area or fume hood. Store it in a cool, dry place away from direct sunlight. In case of spill, use appropriate spill kits and follow local safety regulations. Always refer to the Material Safety Data Sheet (MSDS) for detailed safety information.