Selection of chromatographic separation conditions for reliable monitoring of the transformation of AgNPs/Ag(i) species by HPLC-ICP-MS in surface water and green algae cells
Literature Information
Julita Malejko, Weronika Liszewska, Beata Godlewska-Żyłkiewicz
A new method based on high performance liquid chromatography (HPLC) coupled with inductively coupled plasma mass spectrometry (ICP-MS) has been developed for the speciation analysis of silver nanoparticles (AgNPs) and dissolved silver forms (Ag(I)) in surface water and algal cells. Different compositions of the mobile phase, including surfactants preventing agglomeration/aggregation of AgNPs, pH buffers and complexing agents for silver ions, were carefully investigated to ensure complete elution and stability of silver species during chromatographic analysis. Under the optimal separation conditions (mobile phase: 10 mM sodium dodecyl sulfate, 2 mM citrate buffer, and 2 mM tiopronin), detection limits of 2.0 ng Lā1 for Ag(I), 3.1 ng Lā1 for 10 nm AgNPs, and 2.2 ng Lā1 for 30 nm AgNPs were achieved. A linear relationship was found between the retention time of AgNPs and logarithm of particle diameter up to 40 nm, which can be used for the particle size determination in real samples. The HPLC-ICP-MS method has been successfully applied to study interactions of Ag(I) and citrate-stabilized AgNPs of different nominal diameters (10 nm and 30 nm) with green microalgae Acutodesmus obliquus in river water. The cell-associated fractions of silver were quite similar for all studied forms (silver biosorption was in the range of 77.2ā82.7%). As a result of interaction with the biomass, biosynthesis of small nanoparticles (with a size of 6 nm) from Ag(I) ions and partial dissolution of 10 and 30 nm AgNPs were observed.
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Source Journal
Journal of Analytical Atomic Spectrometry

The Journal of Analytical Atomic Spectrometry (JAAS) is the central journal for publishing innovative research on fundamentals, instrumentation, and methods in the determination, speciation and isotopic analysis of (trace) elements within all fields of application. This includes, but is not restricted to, the most recent progress, developments and achievements in all forms of atomic and elemental detection, isotope ratio determination, molecular analysis, plasma-based analysis and X-ray techniques. The journal welcomes full papers, communications, technical notes, critical and tutorial review articles, editorials, and comments, in addition to the Atomic Spectrometry Updates (ASU) literature reviews that are prepared by an expert panel. Submissions are welcome in the following areas, but note this list reflects the current scope and authors are strongly encouraged to contact the Editorial team if they believe that their work offers potentially new and emerging research relevant to the journal remit: Fundamental studies in the following. New and existing sources for atomic emission, absorption, fluorescence and mass spectrometry and those that provide both atomic and molecular information Sample introduction techniques for solids, liquids, gases Improvements in sensitivity, selectivity, precision, accuracy and/or robustness Isotope ratio measurements, including techniques for improving precision and mass bias correction Single channel and multichannel simultaneous detection systems Chemometrics, statistics, calibration techniques and internal standardisation Theoretical and numerical modelling of fundamental processes related to all of the above methodologies Novel or improved methodologies in areas of application including, but not limited to the following. Biosciences, including elemental, speciation and isotopic analysis in biological systems, immunoassays based on metal-labeled antibodies, bio-imaging, and nanoparticle toxicology Geochemistry Environmental science Materials science, including engineered nanoparticles and quantum dots Metrology, including reference materials Forensic analysis Food and agricultural sciences Energy Archaeometry Molecular analysis. Molecular sources for elemental and isotopic analysis Atomic sources for molecular analysis Atomic and molecular techniques simultaneously used for complementary chemical information All contributions are judged on originality and quality of scientific content, and appropriateness of length to content of new science.











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