Artificial nucleotide codons for enzymatic DNA synthesis

Literature Information

Publication Date 2023-11-15
DOI 10.1039/D3CC04933G
Impact Factor 6.222
Authors

Nazarii Sabat, Andreas Stämpfli, Marie Flamme, Steven Hanlon, Serena Bisagni, Filippo Sladojevich, Kurt Püntener, Marcel Hollenstein


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Abstract

Herein, we report the high-yielding solid-phase synthesis of unmodified and chemically modified trinucleotide triphosphates (dN3TPs). These synthetic codons can be used for enzymatic DNA synthesis provided their scaffold is stabilized with phosphorothioate units. Enzymatic synthesis with three rather than one letter nucleotides will be useful to produce xenonucleic acids (XNAs) and for in vitro selection of modified functional nucleic acids.

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