Fluorescent molecular rotors detect O6-methylguanine dynamics and repair in duplex DNA

Literature Information

Publication Date 2023-12-19
DOI 10.1039/D3CC04782B
Impact Factor 6.222
Authors


View Original

Abstract

Alkylation at the O6 position of guanine is a common and highly mutagenic form of DNA damage. Direct repair of O6-alkylguanines by the “suicide” enzyme O6-methylguanine DNA methyltransferase (MGMT, AGT, AGAT) maintains genome stability and inhibits carcinogenesis. In this study, a fluorescent analogue of thymidine containing trans-stilbene (tsT) is quenched by O6-methylguanine residues in the opposite strand of DNA by molecular dynamics that propagate through the duplex with as much as ∼9 Å of separation. Increased fluorescence of tsT or the cytosine analogue tsC resulting from MGMT-mediated DNA repair were distinguishable from non-covalent DNA–protein binding following protease digest. To our knowledge, this is the first study utilizing molecular rotor base analogues to detect DNA damage and repair activities in duplex DNA.

Related Literature

Preparation of aqueous gel beads coated by lipid bilayers

Andrew Campbell, Pietro Taylor, Olivier J. Cayre, Vesselin N. Paunov

2004-09-23 Communication

DOI: 10.1039/B406941B

Non-interpenetrating honeycomb-like 2D [6,3] network built by a novel trigonal metalloligand

Kyoung-Tae Youm, Seong Huh, Young Jun Park, Sangwoo Park, Moon-Gun Choi, Moo-Jin Jun

2004-09-20 Communication

DOI: 10.1039/B409596K

The Sonogashira coupling reaction catalyzed by ultrafine nickel(0) powder

Pinhua Li, Yicheng Zhang

2004-01-27 Communication

DOI: 10.1039/B314246A

The role of temperature in the synthesis of hybrid inorganic–organic materials: the example of cobalt succinates

Paul M. Forster, Andrea R. Burbank, Carine Livage, Gérard Férey, Anthony K. Cheetham

2004-01-23 Communication

DOI: 10.1039/B311156C

Definitive identification of lead(ii)-amino acid adducts and the solid state structure of a lead–valine complex

Neil Burford, Melanie D. Eelman, Wesley G. LeBlanc, T. Stanley Cameron, Katherine N. Robertson

2004-01-08 Communication

DOI: 10.1039/B311599B

A simple copper salt catalysed the coupling of imidazole with arylboronic acids in protic solvent

Jing-Bo Lan, Li Chen, Xiao-Qi Yu, Jing-Song You, Ru-Gang Xie

2003-11-25 Communication

DOI: 10.1039/B307734A

Parallel nucleic acid recognition by the LNA (locked nucleic acid) stereoisomers β-L-LNA and α-D-LNA; studies in the mirror image world

Nanna K. Christensen, Torsten Bryld, Mads D. Sørensen, Khalil Arar, Jesper Wengel, Poul Nielsen

2003-12-19 Communication

DOI: 10.1039/B312321A

An Fe(iii) wheel with a zwitterionic ligand: the structure and magnetic properties of [Fe(OMe)2(proline)]12[ClO4]12

Abd-Alhakeem H. Abu-Nawwas, Joan Cano, Paul Christian, Talal Mallah, Gopalan Rajaraman, Simon J. Teat, Richard E. P. Winpenny, Yasuhiko Yukawa

2004-01-07 Communication

DOI: 10.1039/B312947K

You might also like

Compound Q&A

What precautions should be taken when handling 2-Chloro-1,2-bis(4-methylphenyl)ethanone (CAS: 71193-32-3)?

When handling 2-Chloro-1,2-bis(4-methylphenyl)ethanone (CAS: 71193-32-3), it is ...

71193-32-32-Chloro-1,2-bis(4-m...
Compound Q&A

What industries use 4-Ethoxy-3-(5-methyl-4-oxo-7-propyl-1,4-dihydroimidazo[5,1-f][1,2,4]triazin-2-yl)benzenesulfonyl chloride (CAS: 224789-26-8)?

4-Ethoxy-3-(5-methyl-4-oxo-7-propyl-1,4-dihydroimidazo[5,1-f][1,2,4]triazin-2-yl...

224789-26-84-Ethoxy-3-(5-methyl...
Compound Q&A

How should Methyl 3-Oxo-4-Androsten-17-Carboxylate (CAS: 2681-55-2) be stored?

Methyl 3-Oxo-4-Androsten-17-Carboxylate (CAS: 2681-55-2) should be stored in a c...

2681-55-2Methyl 3-Oxo-4-Andro...
Compound Q&A

What are the main uses of (R)-3-Amino-4-(3-hexylphenylamino)-4-oxobutylphosphonic acid (CAS: 909725-61-7)?

(R)-3-Amino-4-(3-hexylphenylamino)-4-oxobutylphosphonic acid is primarily used i...

909725-61-7(R)-3-Amino-4-(3-hex...
Compound Q&A

What regulatory guidelines apply to 2-Methyl-2-propanyl 3-amino-3-carbamoyl-1-azetidinecarboxylate (CAS: 1254120-14-3)?

2-Methyl-2-propanyl 3-amino-3-carbamoyl-1-azetidinecarboxylate (CAS: 1254120-14-...

1254120-14-32-Methyl-2-propanyl ...
Compound Q&A

Are there alternatives to (E)-4-(tert-Butoxy)-4-oxobut-2-enoic acid (CAS: 135355-96-3) in synthesis?

There are alternative reagents that can be used in synthesis instead of (E)-4-(t...

135355-96-3(E)-4-(tert-Butoxy)-...
Compound Q&A

What are the physical and chemical properties of [2-(3-Chlorophenyl)-1,3-thiazol-4-yl]methanol (CAS: 121202-20-8)?

[2-(3-Chlorophenyl)-1,3-thiazol-4-yl]methanol (CAS: 121202-20-8) is a crystallin...

121202-20-8[2-(3-Chlorophenyl)-...
166249-17-8Methyl (2S)-[(4S)-2,...
Compound Q&A

What is the market or research trend for 1-Bromo-2-isocyanatoethane (CAS: 42865-19-0)?

The market for 1-Bromo-2-isocyanatoethane (CAS: 42865-19-0) is driven by its use...

42865-19-01-Bromo-2-isocyanato...
Compound Q&A

What are the main uses of 4-Nitro-D-phenylalanine hydrochloride (CAS: 147065-06-3)?

4-Nitro-D-phenylalanine hydrochloride (CAS: 147065-06-3) is primarily used in re...

147065-06-34-Nitro-D-phenylalan...

Source Journal

Chemical Communications

Chemical Communications
CiteScore: 8.6
Self-citation Rate: 4.7%
Articles per Year: 2458

ChemComm publishes urgent research which is of outstanding significance and interest to experts in the field, while also appealing to the journal’s broad chemistry readership. Our communication format is ideally suited to short, urgent studies that are of such importance that they require accelerated publication. Our scope covers all topics in chemistry, and research at the interface of chemistry and other disciplines (such as materials science, nanoscience, physics, engineering and biology) where there is a significant novelty in the chemistry aspects. Major topic areas covered include: Analytical Chemistry Catalysis Chemical Biology and medicinal chemistry Computational Chemistry and Machine Learning Energy and sustainable chemistry Environmental Chemistry Green Chemistry Inorganic Chemistry Materials Chemistry Nanoscience Organic Chemistry Physical Chemistry Polymer Chemistry Supramolecular Chemistry

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.