An ECL sensor combined with a paper electrode for the determination of phenylalanine

Literature Information

Publication Date 2022-10-05
DOI 10.1039/D2AN01340A
Impact Factor 4.616
Authors

Hilal Torul, Elif Çalık Kayiş, I. Hakki Boyaci, Ugur Tamer


View Original

Abstract

Herein, a unique electrochemiluminescence (ECL) sensor combined with a paper electrode was proposed for the detection of phenylalanine (L-Phe) in blood samples. The L-Phe detection was performed by converting L-Phe into ammonia using phenylalanine ammonia-lyase (PAL) enzyme and the ECL signal of Ru(bpy)32+ was produced in combination with ammonia as a co-reactant. In this report, we used AuNP decorated paper electrodes to obtain the ECL signal of Ru-(bpy)32+ in the presence of NH3. The produced ammonia molecules were carried to the working electrode and their contact with the sample was cut off. Since there was no contact between the sample matrix and the electrode, the developed method achieved excellent selectivity. According to experimental data, a linear increase of the ECL signals with the logarithms of varying L-Phe concentrations between 1.5 × 10−2 and 1.211 mM was observed with a correlation coefficient (R2) of 0.9898 and a limit of detection (LOD) of 8.4 × 10−3 mM. The proposed method was efficiently applied for L-Phe detection in reference blood samples and the average recovery was calculated as 96.8%. Furthermore, the HPLC-UV method as a comparison verified that the recovery values provided by the proposed method were acceptable with a similarity of 95.1% for L-Phe detection in a reference blood sample. The results showed that the developed ECL sensor combined with the paper electrode can be considered as a promising unique and powerful technique for a point-of-care diagnosis of PKU.

Related Literature

Well-defined hydrogen and organofunctional polysiloxanes with spiro-fused siloxane backbones

Takahiro Kawatsu, Keita Fuchise, Katsuhiko Takeuchi, Jun-Chul Choi, Kazuhiko Sato, Kazuhiro Matsumoto

2021-03-18 Paper

DOI: 10.1039/D0PY01503B

Improved enantioselectivity in thiol–ene photopolymerization of sulphur-containing polymers with circularly polarized luminescence

Chen-Lu He, Zeyu Feng, Yan Li, Manman Zhou, Liyang Zhao, Sizhen Shan, Mengqiao Wang, Xin Chen, Xi-Sheng Wang, Gang Zou

2021-03-31 Paper

DOI: 10.1039/D1PY00082A

Towards scalable, low dispersity, and dimensionally tunable 2D platelets using living crystallization-driven self-assembly

Charlotte E. Ellis, Tomoya Fukui, Cristina Cordoba, Arthur Blackburn, Ian Manners

2021-05-27 Paper

DOI: 10.1039/D1PY00571E

Structural design of pyrene-functionalized TEMPO-containing polymers for enhanced electrochemical storage performance

Hatice Mutlu, Hongjiao Li, Wolfgang Wenzel

2021-04-06 Paper

DOI: 10.1039/D0PY01421D

Front cover

2021-04-06 Cover

DOI: 10.1039/D1PY90048J

Toughened PLA-b-PCL-b-PLA triblock copolymer based biomaterials: effect of self-assembled nanostructure and stereocomplexation on the mechanical properties

Neha Mulchandani, Kazunari Masutani, Sachin Kumar, Hideki Yamane, Yoshiharu Kimura, Vimal Katiyar

2021-06-18 Paper

DOI: 10.1039/D1PY00429H

Detection and evaluation of polymer–polymer interactions in dilute solutions of associating polymers

Georges M. Pavlov, Anna A. Gosteva, Olga V. Okatova, Olga A. Dommes, Irina I. Gavrilova

2021-03-22 Paper

DOI: 10.1039/D0PY01725F

Mediating covalent crosslinking of single-chain nanoparticles through solvophobicity in organic solvents

Georg M. Scheutz, Justine Elgoyhen, Kyle C. Bentz, Hao Sun, Junpeng Zhao, Daniel A. Savin, Brent S. Sumerlin

2021-07-27 Communication

DOI: 10.1039/D1PY00780G

You might also like

Compound Q&A

What precautions should be taken when handling lithium chloride hydrate (1:1:1) (CAS: 16712-20-2)?

When handling lithium chloride hydrate (1:1:1) (CAS: 16712-20-2), it is importan...

16712-20-2Lithium chloride hyd...
Compound Q&A

Is 4-(4H-1,2,4-Triazol-4-yl)piperidine (CAS: 690261-92-8) safe?

4-(4H-1,2,4-Triazol-4-yl)piperidine is generally considered safe for use in phar...

690261-92-84-(4H-1,2,4-Triazol-...
Compound Q&A

How should waste containing 1,3-Thiazole-2-carboxamide (CAS: 16733-85-0) be handled?

Waste containing 1,3-Thiazole-2-carboxamide (CAS: 16733-85-0) should be collecte...

16733-85-01,3-Thiazole-2-carbo...
Compound Q&A

What regulatory guidelines apply to 5-(Difluoromethyl)-2-fluorobenzonitrile (CAS: 934175-58-3)?

5-(Difluoromethyl)-2-fluorobenzonitrile (CAS: 934175-58-3) is subject to regulat...

934175-58-35-(Difluoromethyl)-2...
Compound Q&A

How is Methyl 3-acetamido-2-thiophenecarboxylate (CAS: 22288-79-5) typically synthesized?

Methyl 3-acetamido-2-thiophenecarboxylate can be synthesized by the reaction of ...

22288-79-5Methyl 3-acetamido-2...
Compound Q&A

What is 4-Isoquinolinecarbonitrile (CAS: 34846-65-6)?

4-Isoquinolinecarbonitrile is a chemical compound with the CAS number 34846-65-6...

34846-65-64-Isoquinolinecarbon...
Compound Q&A

How should Methyl 1H-1,2,3-triazole-4-carboxylate (CAS: 877309-59-6) be stored?

Store Methyl 1H-1,2,3-triazole-4-carboxylate (CAS: 877309-59-6) in a cool, dry p...

877309-59-6Methyl 1H-1,2,3-tria...
Compound Q&A

What regulatory guidelines apply to 6-Bromo[1,3]thiazolo[5,4-b]pyridin-2-amine (CAS: 1160791-13-8)?

6-Bromo[1,3]thiazolo[5,4-b]pyridin-2-amine (CAS: 1160791-13-8) is subject to the...

1160791-13-86-Bromo[1,3]thiazolo...
Compound Q&A

Is (2S,3S)-2-Ammonio-3-(3,4-dihydroxyphenyl)-3-hydroxypropanoate (CAS: 23651-95-8) safe?

(2S,3S)-2-Ammonio-3-(3,4-dihydroxyphenyl)-3-hydroxypropanoate (CAS: 23651-95-8) ...

23651-95-8(2S,3S)-2-Ammonio-3-...
Compound Q&A

What are the physical and chemical properties of 7-bromo-3-methyl-3,4-dihydroquinazolin-4-one (CAS: 1293987-84-4)?

7-Bromo-3-methyl-3,4-dihydroquinazolin-4-one is a solid with a crystalline form....

1293987-84-47-bromo-3-methyl-3,4...

Source Journal

Analyst

Analyst
CiteScore: 7.8
Self-citation Rate: 5.6%
Articles per Year: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.