Bioorthogonal protein labelling enables the study of antigen processing of citrullinated and carbamylated auto-antigens

Literature Information

Publication Date 2021-02-23
DOI 10.1039/D1CB00009H
Impact Factor 0
Authors

Tyrza van Leeuwen, Can Araman, Linda Pieper Pournara, Arieke S. B. Kampstra, Thomas Bakkum, Mikkel H. S. Marqvorsen, Clarissa R. Nascimento, G. J. Mirjam Groenewold, Willemijn van der Wulp, Marcel G. M. Camps, George M. C. Janssen, Peter A. van Veelen, Gerard J. P. van Westen, Antonius P. A. Janssen, Bogdan I. Florea, Herman S. Overkleeft, Ferry A. Ossendorp, René E. M. Toes, Sander I. van Kasteren


View Original

Abstract

Proteolysis is fundamental to many biological processes. In the immune system, it underpins the activation of the adaptive immune response: degradation of antigenic material into short peptides and presentation thereof on major histocompatibility complexes, leads to activation of T-cells. This initiates the adaptive immune response against many pathogens. Studying proteolysis is difficult, as the oft-used polypeptide reporters are susceptible to proteolytic sequestration themselves. Here we present a new approach that allows the imaging of antigen proteolysis throughout the processing pathway in an unbiased manner. By incorporating bioorthogonal functionalities into the protein in place of methionines, antigens can be followed during degradation, whilst leaving reactive sidechains open to templated and non-templated post-translational modifications, such as citrullination and carbamylation. Using this approach, we followed and imaged the post-uptake fate of the commonly used antigen ovalbumin, as well as the post-translationally citrullinated and/or carbamylated auto-antigen vinculin in rheumatoid arthritis, revealing differences in antigen processing and presentation.

Related Literature

Time-resolved photoelectron spectroscopy of adenine and adenosine in aqueous solution

Franziska Buchner, Hans-Hermann Ritze, Jan Lahl, Andrea Lübcke

2013-05-07 Paper

DOI: 10.1039/C3CP51057C

Resonance optical manipulation of nano-objects based on nonlinear optical response

Tetsuhiro Kudo, Hajime Ishihara

2013-07-08 Perspective

DOI: 10.1039/C3CP51969D

A low-cost bio-inspired integrated carbon counter electrode for high conversion efficiency dye-sensitized solar cells

Chunlei Wang, Fanning Meng, Mingxing Wu, Xiao Lin, Tonghua Wang, Jieshan Qiu, Tingli Ma

2013-06-27 Communication

DOI: 10.1039/C3CP52525B

Hydrogen bond network topology in liquid water and methanol: a graph theory approach

Imre Bakó, Ákos Bencsura, Kersti Hermannson, Szabolcs Bálint, Tamás Grósz, Julianna Oláh

2013-08-08 Paper

DOI: 10.1039/C3CP52271G

Contents list

Front/Back Matter

DOI: 10.1039/C3CP90112B

Inside front cover

Cover

DOI: 10.1039/C3CP90085A

Water and water-like liquids: relationships between structure, entropy and mobility

Divya Nayar, Charusita Chakravarty

2013-07-29 Perspective

DOI: 10.1039/C3CP51114F

Functionalized 129Xe as a potential biosensor for membrane fluidity

Matthias Schnurr, Christopher Witte, Leif Schröder

2013-06-07 Communication

DOI: 10.1039/C3CP51227D

Back cover

Cover

DOI: 10.1039/C3CP90116E

You might also like

Compound Q&A

How should waste containing N-Methoxy-N-methyl-1,3-thiazole-5-carboxamide (CAS: 898825-89-3) be handled?

Waste containing N-Methoxy-N-methyl-1,3-thiazole-5-carboxamide (CAS: 898825-89-3...

898825-89-3N-Methoxy-N-methyl-1...
Compound Q&A

How should N-(4-Biphenylyl)dibenzo[b,d]furan-4-amine (CAS: 1318338-47-4) be stored?

N-(4-Biphenylyl)dibenzo[b,d]furan-4-amine should be stored in a tightly sealed c...

1318338-47-4N-(4-Biphenylyl)dibe...
Compound Q&A

What is the market or research trend for 3-Acetamido-5-amino-2,4,6-triiodobenzoic acid (CAS: 1713-07-1)?

The market for 3-Acetamido-5-amino-2,4,6-triiodobenzoic acid (CAS: 1713-07-1) is...

1713-07-13-Acetamido-5-amino-...
Compound Q&A

How should Benzyl 2-O-acetyl-3,4,6-tri-O-benzyl-beta-D-galactopyranoside (CAS: 61820-03-9) be stored?

Benzyl 2-O-acetyl-3,4,6-tri-O-benzyl-beta-D-galactopyranoside (CAS: 61820-03-9) ...

61820-03-9Benzyl 2-O-acetyl-3,...
Compound Q&A

What regulatory guidelines apply to 2-Ethylpiperazine dihydrochloride (CAS: 438050-52-3)?

2-Ethylpiperazine dihydrochloride (CAS: 438050-52-3) is regulated under the Glob...

438050-52-32-Ethylpiperazine di...
Compound Q&A

What regulatory guidelines apply to 1,1'-[1,3-Phenylenebis(methylene)]bis(3-methyl-1H-pyrrole-2,5-dione) (CAS: 119462-56-5)?

1,1'-[1,3-Phenylenebis(methylene)]bis(3-methyl-1H-pyrrole-2,5-dione) (CAS: 11946...

119462-56-51,1'-[1,3-Phenyleneb...
Compound Q&A

Are there alternatives to 5-Fluoro-2-(1-pyrrolidinyl)pyridine (CAS: 1287217-79-1) in synthesis?

Several alternatives can be used in the synthesis of 5-Fluoro-2-(1-pyrrolidinyl)...

1287217-79-15-Fluoro-2-(1-pyrrol...
Compound Q&A

What precautions should be taken when handling 6-Bromoimidazo[1,2-a]pyridin-8-amine (CAS: 676371-00-9)?

When handling 6-Bromoimidazo[1,2-a]pyridin-8-amine, it is important to wear appr...

676371-00-96-Bromoimidazo[1,2-a...
Compound Q&A

Are there alternatives to (2S,4R)-4-(4-Nitrobenzyl)pyrrolidine-2-carboxylic acid hydrochloride (CAS: 1049740-22-8) in synthesis?

Alternatives to (2S,4R)-4-(4-Nitrobenzyl)pyrrolidine-2-carboxylic acid hydrochlo...

1049740-22-8(2S,4R)-4-(4-Nitrobe...

Source Journal

RSC Chemical Biology

RSC Chemical Biology
CiteScore: 0
Self-citation Rate: 0%
Articles per Year: 0

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.