Evaluation of top-down mass spectrometry and ion-mobility spectroscopy as a means of mapping protein-binding motifs within heparin chains
Literature Information
Yunlong Zhao, Igor A. Kaltashov
Identifying structural elements within heparin (as well as other glycosaminoglycan) chains that enable their interaction with a specific client protein remains a challenging task due to the high degree of both intra- and inter-chain heterogeneity exhibited by this polysaccharide. The new experimental approach explored in this work is based on the assumption that the heparin chain segments bound to the protein surface will be less prone to collision-induced dissociation (CID) in the gas phase compared to the chain regions that are not involved in binding. Facile removal of the unbound chain segments from the protein/heparin complex should allow the length and the number of sulfate groups within the protein-binding segment of the heparin chain to be determined by measuring the mass of the truncated heparin chain that remains bound to the protein. Conformational integrity of the heparin-binding interface on the protein surface in the course of CID is ensured by monitoring the evolution of collisional cross-section (CCS) of the protein/heparin complexes as a function of collisional energy. A dramatic increase in CCS signals the occurrence of large-scale conformational changes within the protein and identifies the energy threshold, beyond which relevant information on the protein-binding segments of heparin chains is unlikely to be obtained. Testing this approach using a 1 : 1 complex formed by a recombinant form of an acidic fibroblast growth factor (FGF-1) and a synthetic pentasaccharide GlcNS,6S-GlcA-GlcNS,3S,6S-IdoA2S-GlcNS,6S-Me as a model system indicated that a tri-saccharide fragment is the minimal-length FGF-binding segment. Extension of this approach to a decameric heparin chain (dp10) allowed meaningful binding data to be obtained for a 1 : 1 protein/dp10 complex, while the ions representing the higher stoichiometry complex (2 : 1) underwent dissociation via asymmetric charge partitioning without generating truncated heparin chains that remain bound to the protein.
Related Literature
In situ temperature measurements of reaction spaces under microwave irradiation using photoluminescent probes
Taishi Ano, Fuminao Kishimoto, Ryo Sasaki, Shuntaro Tsubaki, Masato M. Maitani, Eiichi Suzuki, Yuji Wada
DOI: 10.1039/C6CP02034H
Transfer of complexed and dissociated ionic species at soft interfaces: a voltammetric study of chemical kinetic and diffusional effects
Eduardo Laborda, José Manuel Olmos, Ángela Molina
DOI: 10.1039/C6CP00780E
CO adsorption on the GaPd() surface: a comparative DFT study using different functionals
S. V. Levchenko
DOI: 10.1039/C6CP01820C
Correlation of the depletion layer with the Helmholtz layer in the anatase TiO2–H2O interface via molecular dynamics simulations
Lixia Sang, Yudong Zhang, Jun Wang, Yangbo Zhao, Yi-tung Chen
DOI: 10.1039/C6CP01990K
Endohedral charge-transfer complex Ca@B37−: stabilization of a B373− borospherene trianion by metal-encapsulation
Hai-Ru Li, Wen-Juan Tian, Hai-Gang Lu, Hua-Jin Zhai, Si-Dian Li
DOI: 10.1039/C6CP02369J
The internal-strain tensor of crystals for nuclear-relaxed elastic and piezoelectric constants: on the full exploitation of its symmetry features
DOI: 10.1039/C6CP01971D
Collision induced state-to-state energy transfer dynamics between the 2u (1D2) and 2g (1D2) ion-pair states of I2
Shoma Hoshino, Yukio Nakano, Mitsunori Araki, Takashi Ishiwata, Koichi Tsukiyama
DOI: 10.1039/C6CP00222F
Distance measurements between paramagnetic ligands bound to parallel stranded guanine quadruplexes
V. A. Szalai
DOI: 10.1039/C6CP01121G
Hydrostatic pressure effect on charge transport properties of phenacene organic semiconductors
Thao P. Nguyen
DOI: 10.1039/C6CP00127K
You might also like
How should waste containing 4-Bromo-3-methyl-2-thiophenecarboxylic acid (CAS: 265652-39-9) be handled?
Waste containing 4-Bromo-3-methyl-2-thiophenecarboxylic acid (CAS: 265652-39-9) ...
What industries use (2S,5S,2'S,5'S)-1,1'-(1,2-Ethanediyl)bis(2,5-dimethylphospholane) (CAS: 136779-26-5)?
(2S,5S,2'S,5'S)-1,1'-(1,2-Ethanediyl)bis(2,5-dimethylphospholane) is primarily u...
What industries use Ethyl 2-(2-bromo-5-fluorophenyl)acetate (CAS: 1214910-61-8)?
Ethyl 2-(2-bromo-5-fluorophenyl)acetate (CAS: 1214910-61-8) is used in the pharm...
How is 4-Methyl-2-benzofuran-1,3-dione (CAS: 4792-30-7) typically synthesized?
4-Methyl-2-benzofuran-1,3-dione (CAS: 4792-30-7) can be synthesized through seve...
What industries use 4,6-Dichloroquinoline-3-carbonitrile (CAS: 936498-04-3)?
4,6-Dichloroquinoline-3-carbonitrile (CAS: 936498-04-3) is used in the pharmaceu...
What are the main uses of Chloro[tris(para-trifluoromethylphenyl)phosphine]gold(I) (CAS: 385815-83-8)?
Chloro[tris(para-trifluoromethylphenyl)phosphine]gold(I) is primarily used in or...
Is 2-Bromo-5-nitrofuran (CAS: 823-73-4) safe?
2-Bromo-5-nitrofuran (CAS: 823-73-4) is generally considered safe when handled w...
How should 5-Bromo-2,3,4-trifluorobenzoic acid (CAS: 212631-85-1) be stored?
5-Bromo-2,3,4-trifluorobenzoic acid should be stored in a cool, dry place away f...
What are the main uses of Zinc bis(aminoacetate) (CAS: 7214-08-6)?
Zinc bis(aminoacetate) (CAS: 7214-08-6) is primarily used in the pharmaceutical ...
How should Adamantan-1-ylmethanol (CAS: 770-71-8) be stored?
Adamantan-1-ylmethanol should be stored in a cool, dry, and well-ventilated plac...
Source Journal
Analyst

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.











![[4-(Hydroxymethyl)phenyl]acetic acid structure [4-(Hydroxymethyl)phenyl]acetic acid structure](https://static.chemtradehub.com/structs/734/73401-74-8-5a54.webp)

![2,5-Furandione, dihydro-3-[3-(triethoxysilyl)propyl]- structure 2,5-Furandione, dihydro-3-[3-(triethoxysilyl)propyl]- structure](https://static.chemtradehub.com/structs/936/93642-68-3-3b4b.webp)
![(1S,2R,4S)-1,7,7-Trimethylbicyclo[2.2.1]heptan-2-ol structure (1S,2R,4S)-1,7,7-Trimethylbicyclo[2.2.1]heptan-2-ol structure](https://static.chemtradehub.com/structs/464/464-45-9-f88b.webp)