Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman spectroscopy

Literature Information

Publication Date 2014-11-19
DOI 10.1039/C4AN01810A
Impact Factor 4.616
Authors

Abida Naemat, Hany M. Elsheikha, Alaa Al-sandaqchi, Kenny Kong, Adrian Ghita, Ioan Notingher


View Original

Abstract

Label-free imaging using Raman micro-spectroscopy (RMS) was used to characterize the spatio-temporal molecular changes of T. gondii tachyzoites and their host cell microenvironment. Raman spectral maps were recorded from isolated T. gondii tachyzoites and T. gondii-infected human retinal cells at 6 h, 24 h and 48 h post-infection. Principal component analysis (PCA) of the Raman spectra of paraformaldehyde-fixed infected cells indicated a significant increase in the amount of lipids and proteins in the T. gondii tachyzoites as the infection progresses within host cells. These results were confirmed by experiments carried out on live T. gondii-infected cells and were correlated with an increase in the concentration of proteins and lipids required for the replication of this intracellular pathogen. These findings demonstrate the potential of RMS to characterize time- and spatially-dependent molecular interactions between intracellular pathogens and the host cells. Such information may be useful for discovery of pharmacological targets or screening compounds with potential neuro-protective activity for eminent effects of changes in brain infection control practices.

Related Literature

A fast track strategy toward highly functionalized dendrimers with different structural layers: an “onion peel approach”

Rishi Sharma, Issan Zhang, Leïla Abbassi, Rabindra Rej, Dusica Maysinger, René Roy

2015-01-16 Communication

DOI: 10.1039/C4PY01761G

High performance thin film transistors based on bi-thieno[3,4-c]pyrrole-4,6-dione-containing copolymers: tuning the face-on and edge-on packing orientations

Xiaolan Qiao, Qinghe Wu, Hongzhuo Wu, Deliang Wang, Hongxiang Li

2015-12-29 Paper

DOI: 10.1039/C5PY01995H

Syndioselective ring-opening polymerization and copolymerization of trans-1,4-cyclohexadiene carbonate mediated by achiral metal- and organo-catalysts

Abdou Khadri Diallo, Evgueni Kirillov, Martine Slawinski, Jean-Michel Brusson, Sophie M. Guillaume, Jean-François Carpentier

2015-01-05 Paper

DOI: 10.1039/C4PY01713G

Contents list

Front/Back Matter

DOI: 10.1039/C5PY90033F

Reversible and “fingerprint” fluorescence differentiation of organic amine vapours using a single conjugated polymer probe

Yanyan Fu, Wei Xu, Qingguo He, Defeng Zhu, Huimin Cao, Jiangong Cheng

2015-01-27 Communication

DOI: 10.1039/C4PY01793E

Using Hansen solubility parameters to predict the dispersion of nano-particles in polymeric films

M. Andersson, J. Engström, P. Restorp

2016-01-21 Paper

DOI: 10.1039/C5PY01935D

Glycopolymer-based nanoparticles: synthesis and application

Xiao Li, Gaojian Chen

2015-01-19 Review Article

DOI: 10.1039/C4PY01740D

Facile synthesis of cyclosiloxane-based polymers for hybrid film formation

Ali Demirci, Shunsuke Yamamoto, Jun Matsui, Tokuji Miyashita, Masaya Mitsuishi

2015-02-19 Paper

DOI: 10.1039/C5PY00018A

Discrete copper(ii)-formate complexes as catalytic precursors for photo-induced reversible deactivation polymerization

Vasiliki Nikolaou, Francesca Brandford-Adams, Richard Whitfield, Glen R. Jones, Gabit Nurumbetov

2015-10-20 Paper

DOI: 10.1039/C5PY01578B

Azide-based heterobifunctional poly(ethylene oxide)s: NaN3-initiated “living” polymerization of ethylene oxide and chain end functionalizations

Sera Yang, Youn Kim, Hyeong Cheol Kim, Abu B. Siddique, Gyusaang Youn, Hyun Jun Kim, Hyeon Jong Park, Jae Yeol Lee, Sehoon Kim, Jungahn Kim

2015-11-02 Paper

DOI: 10.1039/C5PY01444A

You might also like

Compound Q&A

Is 2-(2-chloroacetamido)-3-phenylpropanoic acid (CAS: 7765-11-9) safe?

2-(2-Chloroacetamido)-3-phenylpropanoic acid (CAS: 7765-11-9) is generally consi...

7765-11-92-(2-chloroacetamido...
Compound Q&A

Is 2-(Benzyloxy)-5-bromobenzoic acid (CAS: 62176-31-2) safe?

2-(Benzyloxy)-5-bromobenzoic acid can be handled safely if appropriate precautio...

62176-31-22-(Benzyloxy)-5-brom...
Compound Q&A

What is (4-Methyl-1,2,5-oxadiazol-3-yl)methanamine hydrochloride (CAS: 1159825-48-5)?

(4-Methyl-1,2,5-oxadiazol-3-yl)methanamine hydrochloride is a chemical compound ...

1159825-48-5(4-Methyl-1,2,5-oxad...
Compound Q&A

What is 2-(5-Hexylthiophen-2-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (CAS: 917985-54-7)?

2-(5-Hexylthiophen-2-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (CAS: 917985-54...

917985-54-72-(5-Hexylthiophen-2...
Compound Q&A

Are there alternatives to 4-(8-Methyl-9H-1,3-dioxolo[4,5-h][2,3]benzodiazepin-5-yl)benzenamine (CAS: 102771-26-6) in synthesis?

While 4-(8-Methyl-9H-1,3-dioxolo[4,5-h][2,3]benzodiazepin-5-yl)benzenamine (CAS:...

102771-26-64-(8-Methyl-9H-1,3-d...
Compound Q&A

What is the market or research trend for tert-butyl 3-hydroxy-4,5,7,8-tetrahydro-2H-pyrazolo[3,4-d]azepine-6-carboxylate (CAS: 851376-80-2)?

The market for tert-butyl 3-hydroxy-4,5,7,8-tetrahydro-2H-pyrazolo[3,4-d]azepine...

851376-80-2tert-butyl 3-hydroxy...
Compound Q&A

How should waste containing 3,5-Diamino-1H-pyrazole-4-carbonitrile (CAS: 6844-58-2) be handled?

Waste containing 3,5-Diamino-1H-pyrazole-4-carbonitrile (CAS: 6844-58-2) should ...

6844-58-23,5-Diamino-1H-pyraz...
Compound Q&A

How is (6-Fluoro-3-pyridinyl)boronic acid (CAS: 351019-18-6) typically synthesized?

(6-Fluoro-3-pyridinyl)boronic acid can be synthesized through the reaction of 6-...

351019-18-6(6-Fluoro-3-pyridiny...
Compound Q&A

What industries use Dibenzyl carbonimidoylbiscarbamate (CAS: 10065-79-9)?

Dibenzyl carbonimidoylbiscarbamate (CAS: 10065-79-9) finds applications in vario...

10065-79-9Dibenzyl carbonimido...
Compound Q&A

What is the market or research trend for (beta,beta,2,3,4,5,6-~2~H_7_)Phenylalanine (CAS: 74228-83-4)?

The market for (beta,beta,2,3,4,5,6-~2~H_7_)Phenylalanine (CAS: 74228-83-4) is g...

74228-83-4(beta,beta,2,3,4,5,6...

Source Journal

Analyst

Analyst
CiteScore: 7.8
Self-citation Rate: 5.6%
Articles per Year: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.