Label free detection of specific protein binding using a microwave sensor

Literature Information

Publication Date 2014-08-04
DOI 10.1039/C4AN00909F
Impact Factor 4.616
Authors

Marcela Salazar-Alvarez, Olga Korostynska, Alex Mason, Ahmed Al-Shamma'a, Jakki C. Cooney, Edmond Magner, Syed A. M. Tofail


View Original

Abstract

The specific binding of streptavidin to biotinylated protein A was demonstrated using a microwave detection system. In control experiments, the degree of non-specific binding was negligible. The method of detection was used to monitor the adsorption of two other proteins, cytochrome c and glucose oxidase, on to the IDE microwave sensor surface. The response of the sensor was also examined on different substrate materials, with detection of protein binding observed obtained on both smooth, conductive (gold) and on rough, insulating (hydroxyapatite) surfaces.

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