Aptamer-based competitive binding assay for one-step quantitation of hepatitis B surface antigen
Literature Information
Publication Date
2014-06-11
DOI
10.1039/C4AN00619D
Impact Factor
4.616
Authors
Sung-Kwan Suh, Seongeun Song, Heung-Bum Oh, Sang Soo Hah
View Original
Abstract
An aptamer-based competitive binding assay for one-step (i.e. no requirement of pre-treatment) quantitation of target molecules of interest has been developed. This method has been successfully employed for the fast and sensitive detection of the surface antigen of the hepatitis B virus (HBsAg). The key features of our method include its low intrinsic background noise, low costs, high resolution, and high sensitivity, enabling the detection of as low as 1.25 mIU mL−1, approximately 40-fold better than that of the most widely used Abbott Architect assay for HBsAg detection, without the tedious extraction and/or washing procedures. Moreover, this assay has better recovery and accuracy than that of conventional competitive binding assay or others for HBsAg quantitation.
Related Literature
A spectroscopic study of the fluorination and defluorination reactions on single-walled carbon nanotubes
Pierre R. Marcoux, Joachim Schreiber, Patrick Batail, Serge Lefrant, Joël Renouard, Guy Jacob, David Albertini, Jean-Yves Mevellec
2002-05-02
Paper
DOI: 10.1039/B109770A
Photophysical properties of [N]phenylenes
C. Dosche, H.-G. Löhmannsröben, A. Bieser, P. I. Dosa, S. Han, M. Iwamoto, A. Schleifenbaum, K. P. C. Vollhardt
2002-04-30
Paper
DOI: 10.1039/B109342H
Probing boundary sites on a Pt/Al2O3 model catalyst by CO2 hydrogenation and in situATR-IR spectroscopy of catalytic solid–liquid interfaces
Davide Ferri, Thomas Bürgi, Alfons Baiker
2002-05-09
Paper
DOI: 10.1039/B111498K
Two ways of looking at Prigogine and Defay's equation
Luís P. N. Rebelo, Vesna Najdanovic-Visak, Zoran P. Visak, M. Nunes da Ponte, Jacobo Troncoso, Claudio A. Cerdeiriña, Luis Romaní
2002-04-25
Paper
DOI: 10.1039/B200292B
Spectral properties of a fluorescing molecule within a spherical metallic nanocavity
Jörg Enderlein
2002-05-07
Paper
DOI: 10.1039/B200502F
Rheo-optical study of colloidal crystals
Tsuneo Okubo, Hiroshi Kimura, Tohru Hatta, Takaharu Kawai
2002-04-22
Paper
DOI: 10.1039/B109963A
Reactions of chemically activated C9H9 species. Part I. The product distribution of the reaction of phenyl radicals with propyne
Luc Vereecken, Holger F. Bettinger, Jozef Peeters
2002-04-22
Paper
DOI: 10.1039/B109452A
Scanning tunneling microscopy study of WS2nanotubes
Luana Scheffer, R. Rosentzveig, A. Margolin, G. Seifert, S. R. Cohen, R. Tenne
2002-05-01
Communication
DOI: 10.1039/B201244H
Solvent distribution in non-ionic surfactant monolayers
R. K. Thomas
2002-05-10
Paper
DOI: 10.1039/B108698G
Doping level change of polythiophene film during its electrochemical growth process
Mingxiao Fu, Gaoquan Shi, Fengen Chen, Xiaoyin Hong
2002-05-07
Paper
DOI: 10.1039/B201041K
You might also like
Compound Q&A
How should waste containing (6-Bromo-2-naphthyl)oxy](dimethyl)(2-methyl-2-propanyl)silane be handled?
Waste containing (6-Bromo-2-naphthyl)oxy](dimethyl)(2-methyl-2-propanyl)silane (...
100751-65-3[(6-Bromo-2-naphthyl...
Compound Q&A
How is 7-Fluoro-4-isoquinolinecarboxylic acid (CAS: 1841081-40-0) typically synthesized?
7-Fluoro-4-isoquinolinecarboxylic acid can be synthesized via a multi-step proce...
1841081-40-07-Fluoro-4-isoquinol...
Compound Q&A
What are the physical and chemical properties of 2,3,5,6-Tetrabromothieno[3,2-b]thiophene (CAS: 124638-53-5)?
2,3,5,6-Tetrabromothieno[3,2-b]thiophene is a crystalline compound with a high m...
124638-53-52,3,5,6-Tetrabromoth...
Compound Q&A
Is 1-[4-(Benzylamino)-7,8-dihydro-5H-pyrano[4,3-d]pyrimidin-2-yl]-2-methyl-1H-indole-4-carboxamide (CAS: 1542705-92-9) safe?
1-[4-(Benzylamino)-7,8-dihydro-5H-pyrano[4,3-d]pyrimidin-2-yl]-2-methyl-1H-indol...
1542705-92-91-[4-(Benzylamino)-7...
Compound Q&A
What is the market or research trend for imidazo[5,1-d]-1,2,3,5-tetrazine-8-carboxylic acid, 3,4-dihydro-3-methyl-4-oxo- (CAS: 113942-30-6)?
The market for imidazo[5,1-d]-1,2,3,5-tetrazine-8-carboxylic acid, 3,4-dihydro-3...
113942-30-6Imidazo[5,1-d]-1,2,3...
Compound Q&A
What is 3-(Triisopropylsilyl)propiolaldehyde (CAS: 163271-80-5)?
3-(Triisopropylsilyl)propiolaldehyde is a synthetic organic compound with the CA...
163271-80-53-(Triisopropylsilyl...
Compound Q&A
What regulatory guidelines apply to 6-Nitro-2H-1,4-benzoxazin-3(4H)-one (CAS: 81721-87-1)?
6-Nitro-2H-1,4-benzoxazin-3(4H)-one (CAS: 81721-87-1) is subject to various regu...
81721-87-16-Nitro-2H-1,4-benzo...
Compound Q&A
How should waste containing (3-Fluorophenyl)(4-{[(2-methyl-2-propanyl)oxy]carbonyl}-1-piperazinyl)acetic acid (CAS: 885272-91-3) be handled?
Waste containing (3-Fluorophenyl)(4-{[(2-methyl-2-propanyl)oxy]carbonyl}-1-piper...
885272-91-3(3-Fluorophenyl)(4-{...
Compound Q&A
What are the physical and chemical properties of N,N'-4,4'-Biphenyldiyldiisonicotinamide (CAS: 55119-40-9)?
N,N'-4,4'-Biphenyldiyldiisonicotinamide is a white crystalline solid with a mole...
55119-40-9N,N'-4,4'-Biphenyldi...
Compound Q&A
What industries use 6-Bromo-8-fluoro-2-quinazolinol (CAS: 1036756-15-6)?
6-Bromo-8-fluoro-2-quinazolinol is primarily used in the pharmaceutical industry...
1036756-15-66-Bromo-8-fluoro-2-q...
Source Journal
Analyst
CiteScore:
7.8
Self-citation Rate:
5.6%
Articles per Year:
653
Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.
Recommended Compounds
Oxalic acid compound with cyclohexylsulfamothioic O-acid (11)
CAS Number:
31895-22-4
Molecular Formula:
C7H13NO4S3
Recommended Suppliers
Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.