Coumarin meets fluorescein: a Förster resonance energy transfer enhanced optical ammonia gas sensor
Literature Information
Publication Date
2014-06-24
DOI
10.1039/C4AN00061G
Impact Factor
4.616
Authors
Marko Dorrestijn, Agathe Camerlo, Špela Korent Urek, Aleksandra Lobnik, Catherine E. Housecroft, Edwin C. Constable, Lukas J. Scherer
View Original
Abstract
This study focuses on the development of an optical ammonia gas sensor, the sensing mechanism of which is based on Förster resonance energy transfer (FRET) between coumarin and fluorescein. The dyes were immobilized into an organically modified silicate matrix during polymerizing methyltriethoxysilane with trifluoropropyltrimethoxysilane on a poly(methyl methacrylate) substrate. The resulting dye-doped xerogel films were exposed to different gaseous ammonia concentrations. A logarithmic decrease of the coumarin fluorescence emission band at 442 nm was observed with increasing gaseous ammonia concentrations, which was due to enhanced FRET between coumarin and fluorescein. The coumarin/fluorescein composition was optimized in order to obtain the best ammonia sensitivity. First experiments in a flow cell gas sensor setup demonstrated a sensitive and reversible response to gaseous ammonia.
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