High performance enzyme fuel cells using a genetically expressed FAD-dependent glucose dehydrogenase α-subunit of Burkholderia cepacia immobilized in a carbon nanotubeelectrode for low glucose conditions

Literature Information

Publication Date 2013-05-07
DOI 10.1039/C3CP51864G
Impact Factor 3.676
Authors

Deby Fapyane, Soo-Jin Lee, Seo-Hee Kang, Du-Hyun Lim, Kwon-Koo Cho, Tae-hyun Nam, Jae-Pyoung Ahn, Jou-Hyeon Ahn, Seon-Won Kim, In Seop Chang


View Original

Abstract

FAD-dependent glucose dehydrogenase (FAD-GDH) of Burkholderia cepacia was successfully expressed in Escherichia coli and subsequently purified in order to use it as an anode catalyst for enzyme fuel cells. The purified enzyme has a low Km value (high affinity) towards glucose, which is 463.8 μM, up to 2-fold exponential range lower compared to glucose oxidase. The heterogeneous electron transfer coefficient (Ks) of FAD-GDH–menadione on a glassy carbon electrode was 10.73 s−1, which is 3-fold higher than that of GOX–menadione, 3.68 s−1. FAD-GDH was able to maintain its native glucose affinity during immobilization in the carbon nanotube and operation of enzyme fuel cells. FAD-GDH–menadione showed 3-fold higher power density, 799.4 ± 51.44 μW cm−2, than the GOX–menadione system, 308.03 ± 17.93 μW cm−2, under low glucose concentration, 5 mM, which is the concentration in normal physiological fluid.

Related Literature

Inside back cover

Cover

DOI: 10.1039/C9CP90139F

Development of a potential energy surface for the O3–Ar system: rovibrational states of the complex

Sangeeta Sur, Ernesto Quintas-Sánchez, Steve A. Ndengué, Richard Dawes

2019-03-12 Paper

DOI: 10.1039/C9CP01044K

A low temperature investigation of the N(2D) + CH4, C2H6 and C3H8 reactions

Michel Dobrijevic

2019-03-06 Paper

DOI: 10.1039/C9CP00798A

The interaction of ethylammonium tetrafluoroborate [EtNH3+][BF4−] ionic liquid on the Li(001) surface: towards understanding early SEI formation on Li metal

Jonathan Clarke-Hannaford, Michael Breedon, Adam S. Best, Michelle J. S. Spencer

2019-04-24 Paper

DOI: 10.1039/C9CP01200A

Mechanisms of fluorescence quenching in prototypical aggregation-induced emission systems: excited state dynamics with TD-DFTB

Thierry Tran, Antonio Prlj, Kun-Han Lin, Daniel Hollas, Clémence Corminboeuf

2019-03-06 Paper

DOI: 10.1039/C9CP00691E

Tailoring the interparticle distance in Langmuir nanoparticle films

Monika Benkovičová, Ana Hološ, Peter Nádaždy, Yuriy Halahovets, Mário Kotlár, Jozef Kollár, Matej Jergel, Jaroslav Mosnáček, Ján Ivančo

2019-04-15 Paper

DOI: 10.1039/C9CP02064K

The multiple dissociation constants of glutathione disulfide: interpreting experimental pH-titration curves with ab initio MD simulations

Vaishali Arunachalam, Anil Kumar Tummanapelli, Sukumaran Vasudevan

2019-04-09 Paper

DOI: 10.1039/C9CP00761J

Delayed vibrational modulation of the solvated GFP chromophore into a conical intersection

Miles A. Taylor, Cheng Chen

2019-04-22 Paper

DOI: 10.1039/C9CP01077G

You might also like

Compound Q&A

How is Ethyl 4-chlorothieno[2,3-b]pyridine-5-carboxylate (CAS: 59713-58-5) typically synthesized?

Ethyl 4-chlorothieno[2,3-b]pyridine-5-carboxylate (CAS: 59713-58-5) can be synth...

59713-58-5Ethyl 4-chlorothieno...
Compound Q&A

What regulatory guidelines apply to 5-Methyl-1H-indole-3-carbaldehyde (CAS: 52562-50-2)?

5-Methyl-1H-indole-3-carbaldehyde (CAS: 52562-50-2) is subject to various regula...

52562-50-25-Methyl-1H-indole-3...
Compound Q&A

What are the physical and chemical properties of (1,3-Dimethyl-2,4-dioxo-1,2,3,4-tetrahydro-5-pyrimidinyl)boronic acid (CAS: 223418-73-3)?

(1,3-Dimethyl-2,4-dioxo-1,2,3,4-tetrahydro-5-pyrimidinyl)boronic acid is a white...

223418-73-3(1,3-Dimethyl-2,4-di...
Compound Q&A

How should waste containing Sulfocostunolide A (CAS: 1016983-51-9) be handled?

Waste containing Sulfocostunolide A (CAS: 1016983-51-9) should be handled with c...

1016983-51-9Sulfocostunolide A
Compound Q&A

What precautions should be taken when handling Murraxocin (CAS: 88478-44-8)?

When handling Murraxocin (CAS: 88478-44-8), ensure proper personal protective eq...

88478-44-8Murraxocin
Compound Q&A

What are the physical and chemical properties of Formvar (CAS: 63148-64-1)?

Formvar (CAS: 63148-64-1) is an alkyd resin characterized by a high molecular we...

63148-64-1Formvar(R)
Compound Q&A

Is (S)-4-benzyl-2-((benzyloxy)methyl)morpholine (CAS: 205242-66-6) safe?

(S)-4-benzyl-2-((benzyloxy)methyl)morpholine is generally safe when handled with...

205242-66-6(S)-4-benzyl-2-((ben...
Compound Q&A

What industries use Methyl 1-(5-bromo-2-pyrimidinyl)cyclopropanecarboxylate (CAS: 1447607-69-3)?

Methyl 1-(5-bromo-2-pyrimidinyl)cyclopropanecarboxylate (CAS: 1447607-69-3) is p...

1447607-69-3Methyl 1-(5-bromo-2-...
Compound Q&A

Is 2-Methyl-1-phenyl-1-propanamine hydrochloride (CAS: 24290-47-9) safe?

2-Methyl-1-phenyl-1-propanamine hydrochloride (CAS: 24290-47-9) is generally con...

24290-47-92-Methyl-1-phenyl-1-...
Compound Q&A

How is 3-(4-Bromophenyl)-2-methylpropanoic acid (CAS: 66735-01-1) typically synthesized?

3-(4-Bromophenyl)-2-methylpropanoic acid is synthesized through a multi-step pro...

66735-01-13-(4-Bromophenyl)-2-...

Source Journal

Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics
CiteScore: 5.5
Self-citation Rate: 10.3%
Articles per Year: 3036

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.