N-Acetylglucosamine biofunctionalized CdSeTe quantum dots as fluorescence probe for specific protein recognition
Literature Information
Publication Date
2012-11-12
DOI
10.1039/C2AN36434D
Impact Factor
4.616
Authors
Fang-Fang Cheng, Yuan-Yuan Shen, Rohit Kumar Rana, Jun-Jie Zhu
View Original
Abstract
Hsp70 proteins are implicated in resistance to chemotherapy in cancers, the detection of which is important for cancer treatment and prognosis. In this work, we report the study on the detection of specific intracellular target protein in fixed cells using GlcNAc-conjugated CdSeTe QDs. The QDs were coupled with Con A via a carbodiimide reaction and then were further assembled with GlcNAc by lectin–carbohydrate interaction between Con A and GlcNAc. The obtained QDs-Con A-GlcNAc conjugates have an emission wavelength at 650 nm that is close to the near-infrared (NIR) regions and a specific recognition for Hsp70. These results show that the QDs-Con A-GlcNAc probe can be a promising tool for direct localization of the Hsp70 protein.
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Analyst
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Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.
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CAS Number:
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Molecular Formula:
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