Two-parameter monitoring in a lab-on-valve manifold, applied to intracellular H2O2 measurements
Literature Information
Ilkka Lähdesmäki, Petr Chocholouš, Andrea D. Carroll, Judy Anderson, Peter S. Rabinovitch, Jaromir Ruzicka
This work introduces, for the first time, simultaneous monitoring of fluorescence and absorbance using Bead Injection in a Lab-on-valve format. The aim of the paper is to show that when the target species, cells immobilized on a stationary phase, are exposed to reagents under well-controlled reaction conditions, dual monitoring yields valuable information. The applicability of this technique is demonstrated by the development of a Bead Injection method for automated measurement of cell density and intracellular hydrogen peroxide.
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