Two-parameter monitoring in a lab-on-valve manifold, applied to intracellular H2O2 measurements

Literature Information

Publication Date 2009-04-27
DOI 10.1039/B822070K
Impact Factor 4.616
Authors

Ilkka Lähdesmäki, Petr Chocholouš, Andrea D. Carroll, Judy Anderson, Peter S. Rabinovitch, Jaromir Ruzicka


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Abstract

This work introduces, for the first time, simultaneous monitoring of fluorescence and absorbance using Bead Injection in a Lab-on-valve format. The aim of the paper is to show that when the target species, cells immobilized on a stationary phase, are exposed to reagents under well-controlled reaction conditions, dual monitoring yields valuable information. The applicability of this technique is demonstrated by the development of a Bead Injection method for automated measurement of cell density and intracellular hydrogen peroxide.

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