![9H-Fluoren-9-ylmethyl {15-[(2,5-dioxo-1-pyrrolidinyl)oxy]-15-oxo-3,6,9,12-tetraoxapentadec-1-yl}carbamate structure](https://static.chemtradehub.com/structs/131/1314378-14-7-4316.webp "9H-Fluoren-9-ylmethyl {15-[(2,5-dioxo-1-pyrrolidinyl)oxy]-15-oxo-3,6,9,12-tetraoxapentadec-1-yl}carbamate structure")
9H-Fluoren-9-ylmethyl {15-[(2,5-dioxo-1-pyrrolidinyl)oxy]-15-oxo-3,6,9,12-tetraoxapentadec-1-yl}carbamate
CAS Number:
1314378-14-7
Molecular Formula:
C30H36N2O10
Electrochemical impedance spectroscopy and surface plasmon resonance studies of DNA hybridization on gold/SiOx interfaces
Literature Information
Publication Date
2008-07-01
DOI
10.1039/B804825H
Impact Factor
4.616
Authors
Maël Manesse, Valerie Stambouli, Rabah Boukherroub
View Original
Abstract
The use of Au/SiOx interfaces for the investigation of DNA hybridization using electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR) simultaneously is demonstrated. Standard glass chemistry was used to link single-stranded DNA (ss-DNA) on aldehyde-terminated Au/SiOx interfaces. The layer thickness and amount of grafted oligonucleotides (ODNs) were calculated from SPR on the basis of a multilayer system of glass/Ti/Au/SiOx/grafted molecule. Capacitance and resistance values of the modified interface before and after hybridization were calculated from EIS data using an equivalent circuit and allowed the affinity rate constant, KA = 4.07 × 105 M−1, to be determined. The EIS results were comparable to those obtained by SPR hybridization kinetics recorded in parallel.
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Source Journal
Analyst
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Articles per Year:
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Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.
Recommended Compounds
2-Methoxy-3-(1-methyl-1H-1,2,4-triazol-3-yl)aniline
CAS Number:
1609394-10-6
Molecular Formula:
C10H12N4O
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