An electrochemiluminescent biosensor for uric acid based on the electrochemiluminescence of bis-[3,4,6-trichloro-2-(pentyloxycarbonyl)-phenyl] oxalate on an ITO electrode modified by an electropolymerized nickel phthalocyanine film

Literature Information

Publication Date 2008-03-20
DOI 10.1039/B716281B
Impact Factor 4.616
Authors

Zhenyu Lin, Zhihuang Chen, Yan Liu, Jian Wang, Guonan Chen


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Abstract

Nickel(II) tetrasulfophthalo-cyanine (NiTSPc) could be electrodeposited onto an indium tin oxide (ITO) electrode to form an electropolymerization film of NiTSPc. The electrochemiluminescent (ECL) behavior of bis-[3,4,6-trichloro-2-(pentyloxy-carbonyl)-phenyl] oxalate (BTPPO) on this modified electrode was investigated in detail. The emission of ECL of BTPPO can be greatly enhanced by hydrogen peroxide at this modified electrode. Thus, a new ECL biosensor for uric acid was developed based on the enzymatic reaction of uric acid in the presence of uricase to produce H2O2. The developed sensor has been used to detect uric acid in real serum samples, and the results compared well with those obtained by the routine method.

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