Phosphoester-transfer mechanism of an RNA-cleaving acidic deoxyribozyme revealed by radioactivity tracking and enzymatic digestion
Literature Information
Srinivas A. Kandadai, William Chiuman, Yingfu Li
A convenient method involving 32P-labeling of an RNA substrate at the cleavage site and subsequent enzymatic digestion of cleavage products via phosphatases reveals that pH4DZ1—an RNA-cleaving deoxyribozyme with optimal activity at pH 4—forms a 5′-cleavage fragment with 2′,3′-cyclic phosphate group and a 3′-cleavage fragment with 5′-OH group.
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