Phosphoester-transfer mechanism of an RNA-cleaving acidic deoxyribozyme revealed by radioactivity tracking and enzymatic digestion

Literature Information

Publication Date 2006-05-09
DOI 10.1039/B604682G
Impact Factor 6.222
Authors

Srinivas A. Kandadai, William Chiuman, Yingfu Li


View Original

Abstract

A convenient method involving 32P-labeling of an RNA substrate at the cleavage site and subsequent enzymatic digestion of cleavage products via phosphatases reveals that pH4DZ1—an RNA-cleaving deoxyribozyme with optimal activity at pH 4—forms a 5′-cleavage fragment with 2′,3′-cyclic phosphate group and a 3′-cleavage fragment with 5′-OH group.

Related Literature

On-surface synthesis of polyazulene with 2,6-connectivity

Qiang Sun, Ian Cheng-Yi Hou, Kristjan Eimre, Carlo A. Pignedoli, Pascal Ruffieux

2019-10-17 Communication

DOI: 10.1039/C9CC07168G

In situ synthesis of fluorescent copper nanoclusters for rapid detection of ascorbic acid in biological samples

Xuan Wang, Caicheng Long, Zixin Jiang, Taiping Qing, Kaiwu Zhang, Peng Zhang, Bo Feng

2019-08-29 Paper

DOI: 10.1039/C9AY01627A

Discrimination of papers used in conservation and restoration by the means of the voltammetry of immobilized microparticles technique

Carolina Mai, Andrea Haba-Martínez, Antonio Doménech-Carbó

2019-08-06 Paper

DOI: 10.1039/C9AY00998A

A succinct strategy for construction of nanoporous ionic organic networks from a pyrylium intermediate

Siying Che, Ilja Popovs, Huimin Luo, Yali Luo, Wei Guo, Hao Chen, Tao Wang, Kecheng Jie, Congmin Wang

2019-10-17 Communication

DOI: 10.1039/C9CC06767A

Stepwise isolation of low-valent, low-coordinate Sn and Pb mono- and dications in the coordination sphere of platinum

Holger Braunschweig, Mehmet Ali Celik, Rian D. Dewhurst, Magdalena Heid, Florian Hupp, Sakya S. Sen

2014-10-15 Edge Article

DOI: 10.1039/C4SC02948H

N-Heterocyclic carbenesversus transition metals for stabilizing phosphinyl radicals

Olivier Back, Bruno Donnadieu, Moritz von Hopffgarten, Susanne Klein, Ralf Tonner, Gernot Frenking, Guy Bertrand

2011-02-18 Edge Article

DOI: 10.1039/C1SC00027F

You might also like

Compound Q&A

What are the main uses of 1H-Indazole-6-carbonitrile (CAS: 141290-59-7)?

1H-Indazole-6-carbonitrile finds applications in pharmaceuticals, where it serve...

141290-59-71H-Indazole-6-carbon...
Compound Q&A

How should waste containing Dioctyl (2E)-2-butenedioate (CAS: 2997-85-5) be handled?

Waste containing Dioctyl (2E)-2-butenedioate (CAS: 2997-85-5) should be collecte...

2997-85-5Dioctyl (2E)-2-buten...
Compound Q&A

What industries use Sodium [(1,2-benzoxazol-3-ylmethyl)sulfonyl]azanide (CAS: 68291-98-5)?

Sodium [(1,2-benzoxazol-3-ylmethyl)sulfonyl]azanide is primarily used in pharmac...

68291-98-5Sodium [(1,2-benzoxa...
Compound Q&A

Are there alternatives to Dimethyl 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2,6-pyridinedicarboxylate (CAS: 741709-66-0) in synthesis?

Dimethyl 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2,6-pyridinedicarboxyla...

741709-66-0Dimethyl 4-(4,4,5,5-...
Compound Q&A

How should waste containing 2-Fluoro-6-hydrazinopyridine (CAS: 80714-39-2) be handled?

Waste containing 2-Fluoro-6-hydrazinopyridine (CAS: 80714-39-2) should be manage...

80714-39-22-Fluoro-6-hydrazino...
Compound Q&A

What is 6-Formyl-2-pyridinecarboxylic acid (CAS: 499214-11-8)?

6-Formyl-2-pyridinecarboxylic acid is an organic compound with the molecular for...

499214-11-86-Formyl-2-pyridinec...
900874-91-13-(3,4-dimethoxyphen...
Compound Q&A

How is 9H-Tribenzo[b,d,f]azepine (CAS: 29875-73-8) typically synthesized?

9H-Tribenzo[b,d,f]azepine is typically synthesized via a multi-step process invo...

29875-73-89H-Tribenzo[b,d,f]az...
Compound Q&A

How is 1-Cyclopropyl-7-ethoxy-6-fluoro-8-methoxy-4-oxo-1,4-dihydro-3-quinolinecarboxylic acid (CAS: 1797982-51-4) typically synthesized?

1-Cyclopropyl-7-ethoxy-6-fluoro-8-methoxy-4-oxo-1,4-dihydro-3-quinolinecarboxyli...

1797982-51-41-Cyclopropyl-7-etho...
Compound Q&A

How should waste containing Methyl 3-oxo-1,2,3,4-tetrahydro-6-quinoxalinecarboxylate (CAS: 671820-52-3) be handled?

Waste containing Methyl 3-oxo-1,2,3,4-tetrahydro-6-quinoxalinecarboxylate (CAS: ...

671820-52-3Methyl 3-oxo-1,2,3,4...

Source Journal

Chemical Communications

Chemical Communications
CiteScore: 8.6
Self-citation Rate: 4.7%
Articles per Year: 2458

ChemComm publishes urgent research which is of outstanding significance and interest to experts in the field, while also appealing to the journal’s broad chemistry readership. Our communication format is ideally suited to short, urgent studies that are of such importance that they require accelerated publication. Our scope covers all topics in chemistry, and research at the interface of chemistry and other disciplines (such as materials science, nanoscience, physics, engineering and biology) where there is a significant novelty in the chemistry aspects. Major topic areas covered include: Analytical Chemistry Catalysis Chemical Biology and medicinal chemistry Computational Chemistry and Machine Learning Energy and sustainable chemistry Environmental Chemistry Green Chemistry Inorganic Chemistry Materials Chemistry Nanoscience Organic Chemistry Physical Chemistry Polymer Chemistry Supramolecular Chemistry

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.