QCM/HCC as a platform for detecting the binding of warfarin to an immobilized film of human serum albumin
Literature Information
G. Zilberman
Quartz crystal microbalance/heat conduction calorimetry (QCM/HCC) is a new measurement technology that has been used to monitor simultaneously the mass and motional resistance of a thin film in conjunction with the heat flow produced by a chemical change in the film initiated by reaction with a gas. In this work we examine the applicability of the QCM/HCC in detecting chemical changes at the solution/thin film interface. Human serum albumin (HSA) was bound to the gold electrode of a 5 MHz AT-cut quartz resonator using three types of linkers and then exposed to buffered solutions of the anticoagulant drug warfarin. Changes in resonator frequency and motional resistance as well as changes in heat flow produced by warfarin binding to HSA were monitored as a function of the warfarin concentration. Differences in frequency and motional resistance changes depend upon the linker and vary both in magnitude and sign, whereas the integrated heat signal is proportional to the concentration of warfarin and independent of the linker chemistry. Quartz crystal microbalance/heat conduction calorimetry can thus be a useful tool for studying protein–ligand interactions at the solution–surface interface, even though the quartz resonator does not behave as a microbalance.
Recommended Journals

Bioorganic & Medicinal Chemistry

Herald of the Russian Academy of Sciences

Chinese Journal of Chemistry

Heteroatom Chemistry

Medicinal Chemistry Research

Biocatalysis and Biotransformation

Cellulose

Critical Reviews in Solid State and Materials Sciences

Bioorganic & Medicinal Chemistry Letters

NDT & E International
Related Literature
Successive lithiation of acetylene, ethylene and benzene: a comprehensive computational study of large static second hyperpolarizability
Avijit Mondal, Kaushik Hatua, Ria Sinha Roy, Prasanta K. Nandi
DOI: 10.1039/C6CP07845A
Spacer-controlled emission of randomly oriented fluorophores enhanced with surface plasmon-polaritons
Yu. Akimov
DOI: 10.1039/C7CP00151G
Tuning calcium biosensors with a single-site mutation: structural dynamics insights from femtosecond Raman spectroscopy
Sean R. Tachibana, Longteng Tang, Yanli Wang, Weimin Liu
DOI: 10.1039/C6CP08821J
In situ spectroscopic studies on vapor phase catalytic decomposition of dimethyl oxalate
Shweta Hegde, Kalsang Tharpa, Satyanarayana Reddy Akuri, Rakesh K., Ajay Kumar, Raj Deshpande, Sreejit A. Nair
DOI: 10.1039/C6CP07769B
Ordering kinetics of lamella-forming block copolymers under the guidance of various external fields studied by dynamic self-consistent field theory
Xiaomin Wan, Tong Gao, Liangshun Zhang, Jiaping Lin
DOI: 10.1039/C6CP08726D
Evidence for photosensitised hydrogen production from water in the absence of precious metals, redox-mediators and co-catalysts
S. Salzl, M. Ertl, G. Knör
DOI: 10.1039/C6CP07725K
Combined static and dynamic quenching in micellar systems—closed-form integrated rate laws verified using a versatile probe
Tim Kohlmann, Robert Naumann, Christoph Kerzig, Martin Goez
DOI: 10.1039/C6CP08491E
Selective-releasing-affected lubricant mechanism of a self-assembled MoS2/Mo–S–C nanoperiod multilayer film sliding in diverse atmospheres
J. Xu, T. F. He, L. Q. Chai, L. Qiao, X. Q. Zhang, P. Wang, W. M. Liu
DOI: 10.1039/C6CP08356K
Wet chemical etching induced stress relaxed nanostructures on polar & non-polar epitaxial GaN films
Abhiram Gundimeda, Bhasker Gahtori, Nita Dilawar, Ved Varun Aggarwal, Manju Singh, Rajib Rakshit
DOI: 10.1039/C7CP00380C
Reactivity of 4Fe+(CO)n=0–2 + O2: oxidation of CO by O2 at an isolated metal atom
Shaun G. Ard, Oscar Martinez, Jr., Steven A. Brown, Jordan C. Sawyer, P. B. Armentrout, Albert A. Viggiano, Nicholas S. Shuman
DOI: 10.1039/C6CP08703E
You might also like
What are the main uses of 1H-Indazole-6-carbonitrile (CAS: 141290-59-7)?
1H-Indazole-6-carbonitrile finds applications in pharmaceuticals, where it serve...
How should waste containing Dioctyl (2E)-2-butenedioate (CAS: 2997-85-5) be handled?
Waste containing Dioctyl (2E)-2-butenedioate (CAS: 2997-85-5) should be collecte...
What industries use Sodium [(1,2-benzoxazol-3-ylmethyl)sulfonyl]azanide (CAS: 68291-98-5)?
Sodium [(1,2-benzoxazol-3-ylmethyl)sulfonyl]azanide is primarily used in pharmac...
Are there alternatives to Dimethyl 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2,6-pyridinedicarboxylate (CAS: 741709-66-0) in synthesis?
Dimethyl 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2,6-pyridinedicarboxyla...
How should waste containing 2-Fluoro-6-hydrazinopyridine (CAS: 80714-39-2) be handled?
Waste containing 2-Fluoro-6-hydrazinopyridine (CAS: 80714-39-2) should be manage...
What is 6-Formyl-2-pyridinecarboxylic acid (CAS: 499214-11-8)?
6-Formyl-2-pyridinecarboxylic acid is an organic compound with the molecular for...
What is the market or research trend for 3-(3,4-dimethoxyphenyl)-2,5-dimethyl-N-(2-morpholin-4-ylethyl)pyrazolo[1,5-a]pyrimidin-7-amine (CAS: 900874-91-1)?
Research trends for this compound indicate a focus on its potential applications...
How is 9H-Tribenzo[b,d,f]azepine (CAS: 29875-73-8) typically synthesized?
9H-Tribenzo[b,d,f]azepine is typically synthesized via a multi-step process invo...
How is 1-Cyclopropyl-7-ethoxy-6-fluoro-8-methoxy-4-oxo-1,4-dihydro-3-quinolinecarboxylic acid (CAS: 1797982-51-4) typically synthesized?
1-Cyclopropyl-7-ethoxy-6-fluoro-8-methoxy-4-oxo-1,4-dihydro-3-quinolinecarboxyli...
How should waste containing Methyl 3-oxo-1,2,3,4-tetrahydro-6-quinoxalinecarboxylate (CAS: 671820-52-3) be handled?
Waste containing Methyl 3-oxo-1,2,3,4-tetrahydro-6-quinoxalinecarboxylate (CAS: ...
Source Journal
Analyst

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.




