Inversion of enantioselectivity of asymmetric biocatalytic decarboxylation by site-directed mutagenesis based on the reaction mechanism
Literature Information
Yoichiro Ijima, Kaori Matoishi, Yosuke Terao, Nobuhide Doi, Hiroshi Yanagawa, Hiromichi Ohta
The introduction of two mutations (G74C/C188S) based on the estimated reaction mechanism resulted in the inversion of enantioselectivity of arylmalonate decarboxylase, which catalyses the asymmetric decarboxylation of arylmethylmalonate to give optically active arylpropionate.
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