Inversion of enantioselectivity of asymmetric biocatalytic decarboxylation by site-directed mutagenesis based on the reaction mechanism

Literature Information

Publication Date 2005-01-10
DOI 10.1039/B416398B
Impact Factor 6.222
Authors

Yoichiro Ijima, Kaori Matoishi, Yosuke Terao, Nobuhide Doi, Hiroshi Yanagawa, Hiromichi Ohta


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Abstract

The introduction of two mutations (G74C/C188S) based on the estimated reaction mechanism resulted in the inversion of enantioselectivity of arylmalonate decarboxylase, which catalyses the asymmetric decarboxylation of arylmethylmalonate to give optically active arylpropionate.

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