Monitoring the formation of biosilica catalysed by histidine-tagged silicatein
Literature Information
Muhammad Nawaz Tahir, Patrick Théato, Werner E. G. Müller, Heinz C. Schröder, Andreas Janshoff, Jian Zhang, Joachim Huth, Wolfgang Tremel
Surface bound silicatein retains its biocatalytic activity, which was demonstrated by monitoring the immobilisation of silicatein using a histidine-tag chelating anchor and the subsequent biosilicification of SiO2 on surfaces by surface plasmon resonance spectroscopy, atomic force microscopy and scanning electron microscopy.
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