Band spreading control in electrophoresis microchips by localized zeta-potential variation using field-effect

Literature Information

Publication Date 2004-08-10
DOI 10.1039/B407627N
Impact Factor 4.616
Authors

Chia-Yen Lee, Che-Hsin Lin, Lung-Ming Fu


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Abstract

The paper proposes a new technique, which varies the zeta potential along the channel walls in the vicinity of the microchannel corners in such as a way as to minimize the effects of turn-induced dispersion within U-shaped separation channels. The effects of the separation channel geometry, the fluid velocity profile, and boundary control of the zeta potential on the band distribution in the detection area are all discussed within this paper. The results for the folded square U-shaped separation channel indicate that boundary control of the zeta potential by field-effect significantly reduces the band dispersion induced by the 90° turns. Finally, the results confirm that application of the proposed localized zeta potential variation method results in a correction of the band tilting phenomenon and a reduction in the racetrack effect.

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