Microscale determination of purines in tissue samples by capillary liquid chromatography with electrochemical detection
Literature Information
Publication Date
2003-07-14
DOI
10.1039/B303949H
Impact Factor
4.616
Authors
Qing Deng, Lisa M. Kauri, Wei-Jun Qian, Gabriella M. Dahlgren, Robert T. Kennedy
View Original
Abstract
A microscale method for purines involved in intracellular signaling and energy metabolism, including ADP, ATP, cyclic-AMP, NADH and GTP, was developed. The analytes were separated on a fused-silica capillary liquid chromatography column (50 µm inner diameter by 25 cm long) packed with 7 µm reversed-phase particles and detected with a carbon fiber cylinder microelectrode at +1.50 V versus Ag/AgCl reference electrode. With an acetonitrile gradient, the separation was carried out within 15 min. With a 100 nl injection volume, the detection limits varied from 0.9 to 8 fmol depending upon the analyte. The low detection limits make the method suitable for analysis of small tissue samples. As a demonstration of the method, islets of Langerhans were analyzed for their adenosine-related messenger content.
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Source Journal
Analyst
CiteScore:
7.8
Self-citation Rate:
5.6%
Articles per Year:
653
Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.
Recommended Compounds
2-Methyl-2-propanyl 4-(4-bromobenzyl)-1-piperazinecarboxylate
CAS Number:
844891-10-7
Molecular Formula:
C16H23BrN2O2
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