Microscale determination of purines in tissue samples by capillary liquid chromatography with electrochemical detection
Literature Information
Publication Date
2003-07-14
DOI
10.1039/B303949H
Impact Factor
4.616
Authors
Qing Deng, Lisa M. Kauri, Wei-Jun Qian, Gabriella M. Dahlgren, Robert T. Kennedy
View Original
Abstract
A microscale method for purines involved in intracellular signaling and energy metabolism, including ADP, ATP, cyclic-AMP, NADH and GTP, was developed. The analytes were separated on a fused-silica capillary liquid chromatography column (50 µm inner diameter by 25 cm long) packed with 7 µm reversed-phase particles and detected with a carbon fiber cylinder microelectrode at +1.50 V versus Ag/AgCl reference electrode. With an acetonitrile gradient, the separation was carried out within 15 min. With a 100 nl injection volume, the detection limits varied from 0.9 to 8 fmol depending upon the analyte. The low detection limits make the method suitable for analysis of small tissue samples. As a demonstration of the method, islets of Langerhans were analyzed for their adenosine-related messenger content.
Related Literature
Imaging Aβ(1–42) fibril elongation reveals strongly polarised growth and growth incompetent states
Laurence J. Young, Gabriele S. Kaminski Schierle, Clemens F. Kaminski
2017-10-03
Paper
DOI: 10.1039/C7CP03412A
Theoretical design and mechanistic study of the metal-free reduction of CO2 to CO
Gongli Ma, Guoliang Song, Zhen Hua Li
2017-10-05
Paper
DOI: 10.1039/C7CP05538B
A study of the competitive multiple hydrogen bonding effect and its associated excited-state proton transfer tautomerism
Yi-Ting Chen, Pei-Jhen Wu, Chia-Yu Peng, Jiun-Yi Shen, Cheng-Cheng Tsai, Wei-Ping Hu, Pi-Tai Chou
2017-10-03
Paper
DOI: 10.1039/C7CP05002J
Interaction with prefibrillar species and amyloid-like fibrils changes the stiffness of lipid bilayers
Bruno C. Borro, Lucia Parolini, Pietro Cicuta, Vito Foderà, Lorenzo Di Michele
2017-09-27
Communication
DOI: 10.1039/C7CP05339H
Influence of temperature gradients on charge transport in asymmetric nanochannels
Anne M. Benneker, Hans David Wendt, Rob G. H. Lammertink, Jeffery A. Wood
2017-09-25
Paper
DOI: 10.1039/C7CP03281A
Local water sensing: water exchange in bacterial photosynthetic reaction centers embedded in a trehalose glass studied using multiresonance EPR
Anna Nalepa, Marco Malferrari, Wolfgang Lubitz, Anton Savitsky
2017-09-25
Paper
DOI: 10.1039/C7CP03942E
Enhancing the resolution of 1H and 13C solid-state NMR spectra by reduction of anisotropic bulk magnetic susceptibility broadening
Julie L. Calahan, Joseph W. Lubach, Eric J. Munson
2017-09-25
Paper
DOI: 10.1039/C7CP04223J
A detailed experimental and theoretical investigation of the role of cyano groups in the π-bridged acceptor of sensitizers for use in dye-sensitized solar cells (DSCs)
Qinqin Xu, Guang Yang, Yu Ren, Futai Lu, Nuonuo Zhang, Muhammad Qamar, Manlin Yang, Bao Zhang
2017-10-10
Paper
DOI: 10.1039/C7CP04642A
Thermoelectric properties of Se and Zn/Cd/Sn double substituted Co4Sb12 skutterudite compounds
Karthikeyan N., Sivaprasad Ghanta, Jaiganesh G., Anbarasu V., Partha Pratim Jana, Sivakumar K.
2017-09-29
Paper
DOI: 10.1039/C7CP04634K
Density functional theory modeling of C–Au chemical bond formation in gold implanted polyethylene
Andrej Antušek, Martin Blaško, Miroslav Urban, Pavol Noga, Danilo Kisić, Miloš Nenadović, Davor Lončarević, Zlatko Rakočević
2017-10-23
Paper
DOI: 10.1039/C7CP05637K
You might also like
Compound Q&A
How should waste containing 2-Ethyl-4-Methyl-1H-Imidazole-5-Carbaldehyde (CAS: 88634-80-4) be handled?
Waste containing 2-Ethyl-4-Methyl-1H-Imidazole-5-Carbaldehyde (CAS: 88634-80-4) ...
88634-80-42-Ethyl-4-Methyl-1H-...
Compound Q&A
What industries use Triethoxy(octyl)silane (CAS: 1385031-14-0)?
Triethoxy(octyl)silane (CAS: 1385031-14-0) is widely used in the pharmaceuticals...
1385031-14-0Triethoxy(octyl)sila...
Compound Q&A
Are there alternatives to 3-iodo-7-nitro-1H-indazole (CAS: 864724-64-1) in synthesis?
Several alternatives to 3-iodo-7-nitro-1H-indazole (CAS: 864724-64-1) exist in t...
864724-64-13-iodo-7-nitro-1H-in...
Compound Q&A
Are there alternatives to Benzene, bis[(trimethoxysilyl)ethyl] (CAS: 266317-71-9) in synthesis?
Yes, there are alternatives to Benzene, bis[(trimethoxysilyl)ethyl] (CAS: 266317...
266317-71-9Benzene, bis[(trimet...
Compound Q&A
Is Isothiazole-3-carbonitrile (CAS: 1452-17-1) safe?
Isothiazole-3-carbonitrile (CAS: 1452-17-1) is generally considered safe when us...
1452-17-1Isothiazole-3-carbon...
Compound Q&A
Is (3-Chlorophenyl)methanol (CAS: 873-63-2) safe?
(3-Chlorophenyl)methanol (CAS: 873-63-2) is considered low to moderately toxic. ...
873-63-2(3-Chlorophenyl)meth...
Compound Q&A
How is (2S,3S)-2-Hydroxy-3-({[(2-methyl-2-propanyl)oxy]carbonyl}amino)-3-(2-naphthyl)propanoic acid (CAS: 959583-98-3) typically synthesized?
(2S,3S)-2-Hydroxy-3-({[(2-methyl-2-propanyl)oxy]carbonyl}amino)-3-(2-naphthyl)pr...
959583-98-3(2S,3S)-2-Hydroxy-3-...
Compound Q&A
What precautions should be taken when handling Methyl 2-(bromomethyl)-5-methoxybenzoate (CAS: 788081-99-2)?
Proper handling of methyl 2-(bromomethyl)-5-methoxybenzoate requires the use of ...
788081-99-2Methyl 2-(bromomethy...
Compound Q&A
What is 6,8-Dibromoimidazo[1,2-a]pyridine-2-carboxylic acid (CAS: 904805-36-3)?
6,8-Dibromoimidazo[1,2-a]pyridine-2-carboxylic acid (CAS: 904805-36-3) is an aro...
904805-36-36,8-Dibromoimidazo[1...
Compound Q&A
Is 3-Amino-5-bromo-2-pyridinecarbonitrile (CAS: 573675-27-1) safe?
3-Amino-5-bromo-2-pyridinecarbonitrile is considered safe when handled under pro...
573675-27-13-Amino-5-bromo-2-py...
Source Journal
Analyst
CiteScore:
7.8
Self-citation Rate:
5.6%
Articles per Year:
653
Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.
Recommended Compounds
Recommended Suppliers
Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.