A new dark quencher for use in genetic analysis

Literature Information

Publication Date 2003-03-18
DOI 10.1039/B300934C
Impact Factor 6.222
Authors

Jonathan P. May, Lynda J. Brown, Ivo Rudloff, Tom Brown


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Abstract

A novel, long-wavelength, non-fluorescent quencher (LQ), based on 1,4-diaminoanthraquinone, has been incorporated at the 3′ and 5′-termini of oligonucleotides. The quencher has been used in Molecular beacons, efficiently quenching the long wavelength fluorophore, Cy5.

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