Ca2+ enhanced catalytic NADH oxidation: a coupled 31P-NMR and electrochemistry study

Literature Information

Publication Date 2003-04-17
DOI 10.1039/B212605B
Impact Factor 3.676
Authors

Nicolas Mano, Alexander Kuhn, Stéphane Menu, Erick J. Dufourc


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Abstract

The Ca2+-promoted 500% increase in activity of nitrofluorenone modified electrodes towards NADH oxidation is studied by 31P-NMR. The interaction of Ca2+ with NADH on the one hand and with the redox mediator on the other hand, results in a conformational change of the coenzyme. This event occurs concomitantly with the observed current increase. A three-member complex, in which Ca2+ bridges a NADH phosphate and the carboxyl group of the mediator is proposed as the molecular key for the potentiation of the electrocatalytic current.

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Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics
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