Probing the mechanism of a fungal glycosyltransferase essential for cell wall biosynthesis. UDP-Chitobiose is not a substrate for chitin synthase

Literature Information

Publication Date 2002-12-05
DOI 10.1039/B208953J
Impact Factor 3.876
Authors

Robert Chang, Adam R. Yeager, Nathaniel S. Finney


View Original

Abstract

Chitin synthase is responsible for the biosynthesis of chitin, an essential component of the fungal cell wall. There is a long-standing question as to whether “processive” transferases such as chitin synthase operate in the same manner as non-processive transferases. The question arises from analysis of the polysaccharide structure – in chitin, for instance, each sugar residue is rotated ≈180° relative to the preceding sugar in the chain. This requires that the enzyme account for the alternating “up/down” configuration during biosynthesis. An enzyme with a single active site, analogous to the non-processive transferases – would have to accommodate a distorted glycosidic linkage at every other synthetic step. An alternative proposal is that the enzyme might assemble the disaccharide donor, addressing the “up/down” conformational problem prior to polymer synthesis. We present compelling evidence that this latter hypothesis is incorrect.

Related Literature

Chitosan: an efficient recyclable catalyst for transamidation of carboxamides with amines under neat conditions

Sadu Nageswara Rao, Darapaneni Chandra Mohan, Subbarayappa Adimurthy

2014-07-28 Communication

DOI: 10.1039/C4GC01402B

Sustainable chromatography (an oxymoron?)

Emily A. Peterson, Barry Dillon, Izzat Raheem, Paul Richardson, Daniel Richter, Rachel Schmidt, Helen F. Sneddon

2014-07-15 Perspective

DOI: 10.1039/C4GC00615A

Inside front cover

Cover

DOI: 10.1039/C4GC90036G

Rational investigations in the ring opening of cyclic carbonates by amines

L. Jean-Gérard, R. Auvergne, D. Benazet, S. Caillol, B. Andrioletti

2014-07-16 Paper

DOI: 10.1039/C4GC01032A

General and selective reductive amination of carbonyl compounds using a core–shell structured Co3O4/NGr@C catalyst

Tobias Stemmler, Felix A. Westerhaus, Annette-Enrica Surkus, Marga-Martina Pohl, Kathrin Junge, Matthias Beller

2014-07-29 Communication

DOI: 10.1039/C4GC00536H

Selective conversion of lignin in corncob residue to monophenols with high yield and selectivity

Zhicheng Jiang, Ting He, Jianmei Li, Changwei Hu

2014-06-30 Paper

DOI: 10.1039/C4GC00620H

Development of a tripartite solvent blend for sustainable chromatography

Florence M. Chardon, Nicole Blaquiere, Georgette M. Castanedo, Stefan G. Koenig

2014-07-11 Communication

DOI: 10.1039/C4GC00884G

Tin-free catalysts for the production of aliphatic thermoplastic polyurethanes

Yves Schellekens, Bert Van Trimpont, Pieter-Jan Goelen, Koen Binnemans, Mario Smet, Marie-Anne Persoons, Dirk De Vos

2014-07-21 Paper

DOI: 10.1039/C4GC00873A

Cobalt catalysts: very efficient for hydrogenation of biomass-derived ethyl levulinate to gamma-valerolactone under mild conditions

Huacong Zhou, Jinliang Song, Honglei Fan, Binbin Zhang, Yingying Yang, Jiayin Hu, Qinggong Zhu, Buxing Han

2014-06-05 Paper

DOI: 10.1039/C4GC00482E

You might also like

Compound Q&A

How should 2-Methylbenzene-1,4-diamine dihydrochloride (CAS: 615-45-2) be stored?

2-Methylbenzene-1,4-diamine dihydrochloride (CAS: 615-45-2) should be stored in ...

615-45-22-Methylbenzene-1,4-...
Compound Q&A

Is (1S,4S)-2,5-Diazabicyclo[2.2.1]heptane dihydrobromide (CAS: 132747-20-7) safe?

(1S,4S)-2,5-Diazabicyclo[2.2.1]heptane dihydrobromide is generally considered sa...

132747-20-7(1S,4S)-2,5-Diazabic...
Compound Q&A

What industries use (6-Chloropyridazin-3-YL)methanamine (CAS: 871826-15-2)?

(6-Chloropyridazin-3-YL)methanamine finds applications in the pharmaceutical ind...

871826-15-2(6-Chloropyridazin-3...
Compound Q&A

What are the main uses of 2-Fluoro-3-methylphenol (CAS: 77772-72-6)?

2-Fluoro-3-methylphenol is primarily used in the synthesis of pharmaceuticals, p...

77772-72-62-Fluoro-3-methylphe...
Compound Q&A

What precautions should be taken when handling 3-Methoxy-4-nitrobenzonitrile (CAS: 177476-75-4)?

When handling 3-Methoxy-4-nitrobenzonitrile, it is important to wear appropriate...

177476-75-43-Methoxy-4-nitroben...
Compound Q&A

What precautions should be taken when handling 1,3-Oxazolo[4,5-b]pyridine-2(3H)-thione (CAS: 211949-57-4)?

When handling 1,3-Oxazolo[4,5-b]pyridine-2(3H)-thione (CAS: 211949-57-4), it is ...

211949-57-4[1,3]Oxazolo[4,5-b]p...
Compound Q&A

What regulatory guidelines apply to 4-Ethynylbenzamide (CAS: 90347-86-7)?

4-Ethynylbenzamide (CAS: 90347-86-7) falls under various regulatory guidelines i...

90347-86-74-Ethynylbenzamide
Compound Q&A

What are the main uses of 3-(2-Ethylphenyl)-2-thioxo-4-imidazolidinone (CAS: 186822-57-1)?

3-(2-Ethylphenyl)-2-thioxo-4-imidazolidinone is primarily used as an intermediat...

186822-57-13-(2-Ethylphenyl)-2-...
Compound Q&A

What is (2-Fluoro-6-methoxyphenyl)acetic acid (CAS: 500912-19-6)?

(2-Fluoro-6-methoxyphenyl)acetic acid, also known as 4-fluoro-3-methoxybenzoic a...

500912-19-6(2-Fluoro-6-methoxyp...
Compound Q&A

What is the market or research trend for 2-[4-(Hydroxymethyl)phenoxy]ethanol (CAS: 102196-18-9)?

Market trends for 2-[4-(Hydroxymethyl)phenoxy]ethanol (CAS: 102196-18-9) indicat...

102196-18-92-[4-(Hydroxymethyl)...

Source Journal

Organic & Biomolecular Chemistry

Organic & Biomolecular Chemistry
CiteScore: 3.4
Self-citation Rate: 10.3%
Articles per Year: 1041

Organic & Biomolecular Chemistry (OBC) publishes original and high impact research and reviews in organic chemistry. We welcome research that shows new or significantly improved protocols or methodologies in total synthesis, synthetic methodology or physical and theoretical organic chemistry as well as research that shows a significant advance in the organic chemistry or molecular design aspects of chemical biology, catalysis, supramolecular and macromolecular chemistry, theoretical chemistry, mechanism-oriented physical organic chemistry, medicinal chemistry or natural products. Articles published in the journal should report new work which makes a highly-significant impact in the field. Routine and incremental work is generally not suitable for publication in the journal. More details about key areas of our scope are below. In all cases authors should include in their article clear rationale for why their research has been carried out.

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.