Two-step mechanism in cationic lipoplex formation as observed by dynamic light scattering, dielectric relaxation and circular dichroism methods
Literature Information
We employed dynamic light scattering, radiowave dielectric spectroscopy and circular dichroism measurements to reveal the structural features of complexes formed in the interaction between DNA and cationic liposomes. Two distinct types of complexes form in these systems, as the charge ratio increases. They are: (i) DNA-coated liposomes, where the DNA binds electrostatically to the outside of the cationic lipid vesicle, (ii) multilamellar complexes followed by rupture and reorganization of the bilayer structure or clusters of DNA-coated vesicles with alternating sheets of DNA and lipid bilayers. The knowledge of the complex morphology occurring in lipid-DNA lipoplex formation could provide insight for the development of efficient artificial delivery systems in genetic engineering and gene therapy.
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Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.











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