Immobilization of horseradish peroxidase on nanometre-scale domains of binary self-assembled monolayers formed from dithiobis-N-succinimidyl propionate and 1-tetradecanethiol on Au(111)

Literature Information

Publication Date 2001-05-16
DOI 10.1039/B101807H
Impact Factor 3.676
Authors

Daisuke Hobara, Yusuke Uno, Takashi Kakiuchi


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Abstract

The nanometre-scale, domain-specific immobilization of horseradish peroxidase (HRP) on binary self-assembled monolayers (SAMs) of dithiobis-N-succinimidyl propionate (DTSP) and 1-tetradecanethiol (TDT) formed on Au(111) has been confirmed by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy. After immersing the Au substrates modified with the binary SAMs into an HRP solution, XPS spectra show significant increases in the peak areas of N(1s) and O(1s), indicating the presence of HRP on the surface. After rinsing of the substrate with a KCl solution only covalently immobilized HRP molecules on the domains composed of DTSP remain on the surface. The enzymatic activity of the immobilized HRP is confirmed using cyclic voltammetry in the presence of catechol as an electron transfer mediator.

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Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics
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Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.

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