Investigation of guanine-rich DNA telomeric structure by a covalently linked BODIPY dye

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Publication Date
DOI 10.1039/A901361J
Impact Factor 3.676
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Abstract

We have investigated the interactions and structures of various guanine-rich oligonucleotides that are covalently linked by a BODIPY dye at the 5′ end. HPLC was used to purify and separate different complexes. A single predominant complex is collected for BODIPY-modified d(T10) and d(G10), while two major complexes are collected at different retention times for the BODIPY-modified d(CGCGT4CGCG) and d(T2G4T2G4) in the HPLC chromatograms during sample preparation. Distinct nonresonant holes in the satellite hole spectra allow us to distinguish different conformational structures with the same BODIPY–oligonucleotide sequence. In addition, our satellite hole results indicate that the coupling between the BODIPY and the thymine residues in the G-rich sequences can affect the yield of the G-rich aggregation. Furthermore, the appearance of new bands in the absorption spectra resulting from BODIPY aggregates may be a useful diagnostic of various G-rich structures at room temperature. This is because the BODIPY aggregates are driven by the association of G-rich sequences.

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Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics
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Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.

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