Neuropeptide FF H-Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH2(CAS: 99566-27-5)

Neuropeptide FF has a molecular weight of approximately 1060.51 Da. It is a water-soluble peptide and does not crystallize easily. It is relatively stable in acidic and basic conditions but susceptible to degradation by proteases. The compound is known for its high reactivity with certain metal ions and other biochemical reagents.

Neuropeptide FF is synthesized using solid-phase peptide synthesis (SPPS) techniques. The process involves the stepwise addition of protected amino acids to an Fmoc-protected resin. Commonly, the Fmoc deprotection is followed by coupling with the next amino acid, typically using HATU or BOP as the coupling reagent. The peptide is then cleaved from the resin using strong acid, such as trifluoroacetic acid (TFA), to yield the final product.

Neuropeptide FF finds applications primarily in the pharmaceutical industry for its role in studying neuroendocrine functions and as a tool for drug discovery. It is also utilized in biochemical research for its effects on neuronal signaling and as a potential therapeutic agent. Additionally, it is used in sensor technology for detecting specific cellular responses and in the development of biosensors.

When handling Neuropeptide FF, it is crucial to wear appropriate personal protective equipment (PPE), including gloves and goggles. The compound should be used in a well-ventilated area or a fume hood to minimize exposure. Spills should be cleaned up with caution using absorbent materials and disposed of according to local regulations. Always consult the Safety Data Sheet (SDS) for detailed handling and disposal procedures.