Evans blue(CAS: 314-13-6)

Evans blue (CAS: 314-13-6) is a water-soluble dye with a molecular weight of approximately 406.14 g/mol. It has a deep blue color and is highly soluble in water. Chemically, it is less reactive and is commonly used in pharmaceuticals and biotechnology for staining cells and tissues. It does not readily react with other compounds under normal conditions.

Evans blue (CAS: 314-13-6) is synthesized from 4,4'-diamino-3,3'-diethylbenzidine and hydroxyquinoline. The reaction involves a condensation step followed by sulfonation, which yields the characteristic blue dye. The process requires careful control of pH and temperature to ensure high yield and selectivity.

Evans blue (CAS: 314-13-6) is widely used in various industries. It is primarily used in pharmaceuticals for cell and tissue staining, in biotechnology for molecular biology applications, and in medical diagnostics. It also has applications in sensor technology and as a reference dye in certain analytical techniques.

When handling Evans blue (CAS: 314-13-6), it is essential to wear appropriate personal protective equipment (PPE), including gloves and lab coat. Work should be conducted in a well-ventilated area or a fume hood to avoid inhalation of dust. Spills should be cleaned up immediately using appropriate chemical spill kits. Always consult the Safety Data Sheet (SDS) for detailed safety information and handling procedures.