IPTG(CAS: 367-93-1)

IPTG, or Isopropyl β-D-1-thiogalactopyranoside, is a synthetic analog of lactose used extensively in molecular biology. Its chemical formula is C9H18O5S, and it is a white crystalline solid that is stable under normal conditions. IPTG is commonly used as an inducer for gene expression in recombinant systems due to its ability to bind to the lac repressor protein and initiate transcription.

IPTG is primarily used in research and biotechnology for inducing the expression of genes in bacteria, particularly in E. coli. It is also employed in pharmaceutical and industrial applications for its role in protein production and as a component in some enzymatic assays. Its stability and non-metabolizable nature make it a preferred inducer in laboratory settings.

IPTG is generally considered safe when handled properly, but it should be treated with care. It is not metabolized by most organisms, so it is not toxic in typical laboratory concentrations. However, direct contact with skin or inhalation may cause irritation, and appropriate safety measures such as gloves and ventilation should be followed during handling.

IPTG should be stored in a cool, dry, and dark place, preferably at 4°C. It is stable for several months when kept in a tightly sealed container away from moisture and light. Avoid exposure to high temperatures or humidity to maintain its chemical integrity and effectiveness for research purposes.