Biotin-PEG2-NHS ester(CAS: 596820-83-6)

Biotin-PEG2-NHS ester (CAS: 596820-83-6) is a crystalline compound with a molecular weight of approximately 421.44 g/mol. It is poorly soluble in water but soluble in organic solvents like DMSO and DMF. This compound is reactive towards amine groups and is commonly used for conjugation reactions. It exhibits good stability in anhydrous organic solvents and air, but hydrolysis may occur in aqueous solutions.

Biotin-PEG2-NHS ester (CAS: 596820-83-6) is synthesized through the reaction of biotin with PEG2-NHS ester. This process typically involves the coupling of biotin to the NHS ester moiety of PEG2 under anhydrous conditions to form the amide bond. The reaction is catalyzed by a base like triethylamine and proceeds with good selectivity and yield, usually 85-95%.

Biotin-PEG2-NHS ester (CAS: 596820-83-6) is widely used in the pharmaceutical industry for target labeling, in the polymer industry for functionalization and cross-linking, in the sensor industry for biosensing applications, and in the semiconductor industry for surface modification. It serves as a versatile linker for attaching biotin to various surfaces and biomolecules.

When handling Biotin-PEG2-NHS ester (CAS: 596820-83-6), it is important to work in a fume hood to avoid inhalation of volatile components. Use personal protective equipment (PPE) such as gloves, goggles, and a lab coat. Dispose of any waste in accordance with local regulations and consult the Safety Data Sheet (SDS) for specific disposal instructions. Avoid contact with skin and eyes, and wash hands thoroughly after use.