Single-cell metabolite profiling enables information-rich classification of lymphocyte types and subtypes

Literature Information

Publication Date 2023-12-06
DOI 10.1039/D3CC05011D
Impact Factor 6.222
Authors

Siyuan Pan, Changyi Liu, Huan Yao, Xingyu Pan, Jinhang Li, Jinlei Yang, Murong Du, Peng Liu, Sichun Zhang, Xinrong Zhang


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Abstract

Lymphocytes play crucial roles in the human immune system; however, detailed metabolite characteristics need to be further investigated. Herein, we propose a lymphocyte classification method based on metabolite profiling at the single-cell level. The percentages of different lymphocyte types were calculated with a low margin of error, confirming that the metabolites could serve as a basis for lymphocyte classification. Furthermore, we analyzed the CD4/CD8 ratio in human peripheral blood to verify the feasibility of this method for the classification of lymphocyte subtypes. The proposed method is expected to be a potential tool for the clinical diagnosis of lymphocyte-related diseases.

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