Single-cell metabolite profiling enables information-rich classification of lymphocyte types and subtypes
Literature Information
Siyuan Pan, Changyi Liu, Huan Yao, Xingyu Pan, Jinhang Li, Jinlei Yang, Murong Du, Peng Liu, Sichun Zhang, Xinrong Zhang
Lymphocytes play crucial roles in the human immune system; however, detailed metabolite characteristics need to be further investigated. Herein, we propose a lymphocyte classification method based on metabolite profiling at the single-cell level. The percentages of different lymphocyte types were calculated with a low margin of error, confirming that the metabolites could serve as a basis for lymphocyte classification. Furthermore, we analyzed the CD4/CD8 ratio in human peripheral blood to verify the feasibility of this method for the classification of lymphocyte subtypes. The proposed method is expected to be a potential tool for the clinical diagnosis of lymphocyte-related diseases.
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