Mechanical characterization of base analogue modified nucleic acids by force spectroscopy
Literature Information
Xavier Viader-Godoy, Yii-Lih Lin, Felix Ritort, Fredrik Westerlund, L. Marcus Wilhelmsson
We use mechanical unfolding of single DNA hairpins with modified bases to accurately assess intra- and intermolecular forces in nucleic acids. As expected, the modification stabilizes the hybridized hairpin, but we also observe intriguing stacking interactions in the unfolded hairpin. Our study highlights the benefit of using base-modified nucleic acids in force-spectroscopy.
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