A more universal and stable method for lactate dehydrogenase isoenzyme test

Literature Information

Publication Date 2019-07-17
DOI 10.1039/C9AY01327J
Impact Factor 2.896
Authors

Peijuan Li, Nanhai Qiu, Li Zhang, Guishu Zhong, Fancai Zeng


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Abstract

As an important enzyme for energy metabolism, lactate dehydrogenase (LDH) and its isoenzymes (LDH isoenzymes) have been extensively studied. LDH zymography is a common method to detect LDH isoenzymes, which offers the special advantage of directly observing 5 isozyme bands in the active state. But it has poor stability, and is difficult to apply for a variety of studies, so it has not been widely utilized. Here we have explored the influence of key factors including the temperature and pH of the running buffer, buffer type, and extraction methods on the detection of LDH isoforms and established a more practical and universal LDH zymography method, which can guarantee a stable and quality result, and be easily applied for different studies.

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Analytical Methods

Analytical Methods
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Analytical Methods welcomes early applications of new analytical and bioanalytical methods and technology demonstrating the potential for societal impact. We require that methods and technology reported in the journal are sufficiently innovative, robust, accurate, and compared to other available methods for the intended application. Developments with interdisciplinary approaches are particularly welcome. Systems should be proven with suitably complex and analytically challenging samples. We encourage developments within, but not limited to, the following technologies and applications: global health, point-of-care and molecular diagnostics biosensors and bioengineering drug development and pharmaceutical analysis applied microfluidics and nanotechnology omics studies, such as proteomics, metabolomics or glycomics environmental, agricultural and food science neuroscience biochemical and clinical analysis forensic analysis industrial process and method development

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