A physical picture for mechanical dissociation of biological complexes: from forces to free energies

Literature Information

Publication Date 2017-01-05
DOI 10.1039/C6CP07508H
Impact Factor 3.676
Authors

Rafael Tapia-Rojo, Juan J. Mazo, Fernando Falo


View Original

Abstract

Single-molecule force spectroscopy is a powerful technique based on the application of controlled forces to macromolecules. In order to relate the measured response of the molecule to its equilibrium and dynamic properties, a suitable physical picture of the involved process is necessary. In this work, we introduce a plausible model for mechanical unbinding of some molecular complexes, based on a novel free energy profile. We combine two standard theoretical frameworks for analyzing force spectroscopy experiments on two protein:protein complexes, obtaining key magnitudes of the underlying free energy profile, which are only understood within the mentioned model. Additionally, we carry out detailed stochastic dynamics simulations to prove the validity of the analysis protocol and the reliability of the free energy profile. Remarkably, we can compare directly the obtained unbinding free energies with the previously known bulk binding free energies, bridging the gap between bulk and single molecule techniques.

Related Literature

Morphology and chemical states of size-selected Ptn clusters on an aluminium oxide film on NiAl(110)

Atsushi Beniya, Noritake Isomura, Hirohito Hirata, Yoshihide Watanabe

2014-07-01 Paper

DOI: 10.1039/C4CP01767F

Improved permeability and selectivity in porous graphene for hydrogen purification

Chengxi Huang, Haiping Wu, Kaiming Deng, Weihua Tang

2014-10-24 Communication

DOI: 10.1039/C4CP04385E

The effect of a detonation nanodiamond coating on the thermal decomposition properties of RDX explosives

Yi Tong, Rui Liu, Tonglai Zhang

2014-06-26 Paper

DOI: 10.1039/C4CP02237H

Di- and tri-oxalkyl derivatives of a boron dipyrromethene (BODIPY) rotor dye in lipid bilayers

Marie Olšinová, Piotr Jurkiewicz, Michal Pozník, Radek Šachl, Tereza Prausová, Martin Hof, Václav Kozmík, Filip Teplý, Jiří Svoboda, Marek Cebecauer

2014-04-10 Paper

DOI: 10.1039/C4CP00888J

More stable and more efficient alternatives of Z-907: carbazole-based amphiphilic Ru(ii) sensitizers for dye-sensitized solar cells

Hammad Cheema, Ashraful Islam, Robert Younts, Bhoj Gautam, Idriss Bedja, Ravindra Kumar Gupta, Liyuan Han, Kenan Gundogdu, Ahmed El-Shafei

2014-10-31 Paper

DOI: 10.1039/C4CP04120H

Enhanced visible light photocatalytic activity of Cu2O via cationic–anionic passivated codoping

Yao Jiang, Hongkunag Yuan

2014-11-05 Paper

DOI: 10.1039/C4CP03631J

Electronic structure investigation of the evanescent AtO+ ion

André Severo Pereira Gomes, Florent Réal, Nicolas Galland, Celestino Angeli, Renzo Cimiraglia, Valérie Vallet

2014-02-20 Paper

DOI: 10.1039/C3CP55294B

Spectro-microscopic photoemission evidence of charge uncompensated areas in Pb(Zr,Ti)O3(001) layers

Dana Georgeta Popescu, Marius Adrian Huşanu, Lucian Trupinǎ, Luminiţa Hrib, Lucian Pintilie, Alexei Barinov, Silvano Lizzit, Paolo Lacovig, Cristian Mihail Teodorescu

2014-11-05 Paper

DOI: 10.1039/C4CP04546G

Interfacial processes studied by coupling electrochemistry at the polarised liquid–liquid interface with in situ confocal Raman spectroscopy

Lukasz Poltorak, Manuel Dossot, Grégoire Herzog, Alain Walcarius

2014-10-28 Paper

DOI: 10.1039/C4CP03254C

You might also like

Compound Q&A

How should waste containing N-Methoxy-N-methyl-1,3-thiazole-5-carboxamide (CAS: 898825-89-3) be handled?

Waste containing N-Methoxy-N-methyl-1,3-thiazole-5-carboxamide (CAS: 898825-89-3...

898825-89-3N-Methoxy-N-methyl-1...
Compound Q&A

How should N-(4-Biphenylyl)dibenzo[b,d]furan-4-amine (CAS: 1318338-47-4) be stored?

N-(4-Biphenylyl)dibenzo[b,d]furan-4-amine should be stored in a tightly sealed c...

1318338-47-4N-(4-Biphenylyl)dibe...
Compound Q&A

What is the market or research trend for 3-Acetamido-5-amino-2,4,6-triiodobenzoic acid (CAS: 1713-07-1)?

The market for 3-Acetamido-5-amino-2,4,6-triiodobenzoic acid (CAS: 1713-07-1) is...

1713-07-13-Acetamido-5-amino-...
Compound Q&A

How should Benzyl 2-O-acetyl-3,4,6-tri-O-benzyl-beta-D-galactopyranoside (CAS: 61820-03-9) be stored?

Benzyl 2-O-acetyl-3,4,6-tri-O-benzyl-beta-D-galactopyranoside (CAS: 61820-03-9) ...

61820-03-9Benzyl 2-O-acetyl-3,...
Compound Q&A

What regulatory guidelines apply to 2-Ethylpiperazine dihydrochloride (CAS: 438050-52-3)?

2-Ethylpiperazine dihydrochloride (CAS: 438050-52-3) is regulated under the Glob...

438050-52-32-Ethylpiperazine di...
Compound Q&A

What regulatory guidelines apply to 1,1'-[1,3-Phenylenebis(methylene)]bis(3-methyl-1H-pyrrole-2,5-dione) (CAS: 119462-56-5)?

1,1'-[1,3-Phenylenebis(methylene)]bis(3-methyl-1H-pyrrole-2,5-dione) (CAS: 11946...

119462-56-51,1'-[1,3-Phenyleneb...
Compound Q&A

Are there alternatives to 5-Fluoro-2-(1-pyrrolidinyl)pyridine (CAS: 1287217-79-1) in synthesis?

Several alternatives can be used in the synthesis of 5-Fluoro-2-(1-pyrrolidinyl)...

1287217-79-15-Fluoro-2-(1-pyrrol...
Compound Q&A

What precautions should be taken when handling 6-Bromoimidazo[1,2-a]pyridin-8-amine (CAS: 676371-00-9)?

When handling 6-Bromoimidazo[1,2-a]pyridin-8-amine, it is important to wear appr...

676371-00-96-Bromoimidazo[1,2-a...
Compound Q&A

Are there alternatives to (2S,4R)-4-(4-Nitrobenzyl)pyrrolidine-2-carboxylic acid hydrochloride (CAS: 1049740-22-8) in synthesis?

Alternatives to (2S,4R)-4-(4-Nitrobenzyl)pyrrolidine-2-carboxylic acid hydrochlo...

1049740-22-8(2S,4R)-4-(4-Nitrobe...

Source Journal

Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics
CiteScore: 5.5
Self-citation Rate: 10.3%
Articles per Year: 3036

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.