Rapid and enzyme-free nucleic acid detection based on exponential hairpin assembly in complex biological fluids
Literature Information
Publication Date
2016-04-20
DOI
10.1039/C6AN00474A
Impact Factor
4.616
Authors
Shan Chen
View Original
Abstract
Herein, we have developed a rapid and enzyme-free nucleic acid amplification detection method that combined the exponential self-assembly of four DNA hairpins and the FRET pair Cy3 and Cy5. This strategy was very ingenious and rapid, and could detect nucleic acids at concentrations as low as 10 pM in 15 min in biological fluids.
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