Controlled growth of immunogold for amplified optical detection of aflatoxin B1
Literature Information
Xu Wang, Reinhard Niessner, Dietmar Knopp
A simple, sensitive and cost-effective method for the analysis of the mycotoxin aflatoxin B1 (AFB1) has been established based on controlled growth of immunogold. AFB1-BSA conjugate modified magnetic beads were employed as capture probe and anti-AFB1 antibody-coated gold colloids were used as detection probe for the immunological recognition of AFB1, as well as for signal transduction. The immune recognition event is converted into the gold enlargement signal which can be quantitatively measured by UV-vis spectroscopy. The autocatalytic enlargement of immunogold was conducted in aqueous solution containing chloroauric acid, hexadecyltrimethylammonium bromide and ascorbic acid. The reaction could be stopped by the addition of sodium thiosulfate. The final absorbance and resonance light scattering intensity were highly dependent on immunogold concentration. After gold enhancement, the sensitivity of the immunoassay was improved and total assay time reduced to 1 h. Under optimized conditions, the linear range and lower detection limit was 0.01–1 ng mL−1 and 7 pg mL−1, respectively. The proposed method offers great promise for sensitive detection of other mycotoxins and organic pollutants.
Related Literature
Enantiomerically pure P-chiral phosphinoselenoic chlorides: inversion of configuration at the P-chirogenic center in the synthesis and reaction of these substances
Tsutomu Kimura, Toshiaki Murai
DOI: 10.1039/B507755A
In situ formation of ligandandcatalyst—application in ruthenium-catalyzed enantioselective reduction of ketones
Patrik Västilä, Jenny Wettergren, Hans Adolfsson
DOI: 10.1039/B505516D
Click-chemistry as an efficient synthetic tool for the preparation of novel conjugated polymers
Dirk Jan V. C. van Steenis, Olivier R. P. David, Gino P. F. van Strijdonck, Jan H. van Maarseveen, Joost N. H. Reek
DOI: 10.1039/B507776A
Solid-state 87Rb NMR signatures for rubidium cations bound to a G-quadruplex
Ramsey Ida, Gang Wu
DOI: 10.1039/B505674H
Effects of nanoconfinement on the morphology and reactivity of organic materials
DOI: 10.1039/B502849N
μ-η3:η4-Lithiocene and η3:η3-zincocene incorporating 1,2-diaza-3,5-diborolyl, a cyclopentadienyl analog
Hanh. V. Ly, Taryn. D. Forster, Darren Maley, Masood Parvez, Roland Roesler
DOI: 10.1039/B508152A
Polymer vesicles formed by amphiphilic diblock copolymers containing a thermotropic liquid crystalline polymer block
Jing Yang, Daniel Lévy, Wei Deng, Patrick Keller, Min-Hui Li
DOI: 10.1039/B508034G
Demethylation of the [Me3Sn(PhN2O2)]4 tetramer into dimeric [Me2Sn(PhN2O2)2]2: a thermally induced methyl-transfer between supramolecules‡
Andrea Deák, Gábor Tárkányi
DOI: 10.1039/B506728F
Lower rim mono-functionalization of resorcinarenes
Frank Hauke, Andrew J. Myles, Julius Rebek Jr.
DOI: 10.1039/B506048F
Control of dark current in photoelectrochemical (TiO2/I−–I3−) and dye-sensitized solar cells
Seigo Ito, Paul Liska, Pascal Comte, Raphaël Charvet, Peter Péchy, Udo Bach, Lukas Schmidt-Mende, Shaik Mohammed Zakeeruddin, Andreas Kay, Mohammad K. Nazeeruddin, Michael Grätzel
DOI: 10.1039/B505718C
You might also like
How should waste containing N-Methoxy-N-methyl-1,3-thiazole-5-carboxamide (CAS: 898825-89-3) be handled?
Waste containing N-Methoxy-N-methyl-1,3-thiazole-5-carboxamide (CAS: 898825-89-3...
How should N-(4-Biphenylyl)dibenzo[b,d]furan-4-amine (CAS: 1318338-47-4) be stored?
N-(4-Biphenylyl)dibenzo[b,d]furan-4-amine should be stored in a tightly sealed c...
What is the market or research trend for 3-Acetamido-5-amino-2,4,6-triiodobenzoic acid (CAS: 1713-07-1)?
The market for 3-Acetamido-5-amino-2,4,6-triiodobenzoic acid (CAS: 1713-07-1) is...
How should Benzyl 2-O-acetyl-3,4,6-tri-O-benzyl-beta-D-galactopyranoside (CAS: 61820-03-9) be stored?
Benzyl 2-O-acetyl-3,4,6-tri-O-benzyl-beta-D-galactopyranoside (CAS: 61820-03-9) ...
What regulatory guidelines apply to 2-Ethylpiperazine dihydrochloride (CAS: 438050-52-3)?
2-Ethylpiperazine dihydrochloride (CAS: 438050-52-3) is regulated under the Glob...
What regulatory guidelines apply to 1,1'-[1,3-Phenylenebis(methylene)]bis(3-methyl-1H-pyrrole-2,5-dione) (CAS: 119462-56-5)?
1,1'-[1,3-Phenylenebis(methylene)]bis(3-methyl-1H-pyrrole-2,5-dione) (CAS: 11946...
Are there alternatives to 5-Fluoro-2-(1-pyrrolidinyl)pyridine (CAS: 1287217-79-1) in synthesis?
Several alternatives can be used in the synthesis of 5-Fluoro-2-(1-pyrrolidinyl)...
What precautions should be taken when handling 1-((2R,3R,4R,5R)-5-((bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-4-hydroxy-3-methoxytetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (CAS: 153631-19-7)?
Proper personal protective equipment (PPE) must be worn when handling this compo...
What precautions should be taken when handling 6-Bromoimidazo[1,2-a]pyridin-8-amine (CAS: 676371-00-9)?
When handling 6-Bromoimidazo[1,2-a]pyridin-8-amine, it is important to wear appr...
Are there alternatives to (2S,4R)-4-(4-Nitrobenzyl)pyrrolidine-2-carboxylic acid hydrochloride (CAS: 1049740-22-8) in synthesis?
Alternatives to (2S,4R)-4-(4-Nitrobenzyl)pyrrolidine-2-carboxylic acid hydrochlo...
Source Journal
Analyst

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.













