Facile immobilization of enzymes on electrospun poly(styrene-alt-maleic anhydride) nanofibres

Literature Information

Publication Date 2011-03-26
DOI 10.1039/C1PY00069A
Impact Factor 5.582
Authors

William J. Cloete, Craig Adriaanse, Pieter Swart, Bert Klumperman


View Original

Abstract

Facile immobilization of horseradish peroxidase (HRP) and glucose oxidase (GOX) was achieved on electrospun nanofibres made of an alternating copolymer of styrene (Sty) and maleic anhydride (MAh). The immobilized enzymes were used to perform a cascade reaction oxidizing D-glucose by GOX to yield H2O2 and employing HRP to catalyze the reaction between the generated H2O2 and Ampliflu Red to yield Resorufin. The average activity of the immobilized HRP was determined to be 21% of that of the free enzyme.

Related Literature

Endonuclease IV cleaves apurinic/apyrimidinic sites in single-stranded DNA and its application for biosensing

Xiang-Juan Kong, Shuang Wu, Yao Cen, Ting-Ting Chen, Ru-Qin Yu, Xia Chu

2016-05-04 Paper

DOI: 10.1039/C6AN00738D

Gadolinium-based MRI contrast agent for the detection of tyrosinase

Hyewon Seo

2019-12-17 Communication

DOI: 10.1039/C9AN02213A

Complexometric titrations: new reagents and concepts to overcome old limitations

Jingying Zhai, Eric Bakker

2016-05-27 Minireview

DOI: 10.1039/C6AN00538A

Electrochromic sensing platform based on steric hindrance effects for CEA detection

Yong Xia, Jing Li, Erkang Wang

2016-04-26 Communication

DOI: 10.1039/C6AN00675B

Mass spectrometric quantification of microRNAs in biological samples based on multistage signal amplification

Xiangtang Li, Rui Xu, Li Pan, Yi-Ming Liu

2019-12-30 Paper

DOI: 10.1039/C9AN02064K

Engineering DNAzyme cascade for signal transduction and amplification

Jing Li, Ke Quan, Yanjing Yang, Xiaohai Yang, Xiangxian Meng, Jin Huang, Kemin Wang

2020-01-10 Paper

DOI: 10.1039/C9AN02003A

Characterization of the furin cleavage motif for HIV-1 trimeric envelope glycoprotein by intact LC-MS analysis

Nicole A. Schneck, Vera B. Ivleva, Cindy X. Cai, Jonathan W. Cooper, Q. Paula Lei

2020-01-02 Communication

DOI: 10.1039/C9AN02098E

Graphene oxide-based electrochemical label-free detection of glycoproteins down to aM level using a lectin biosensor

L. Klukova, J. Filip, S. Belicky, A. Vikartovska, J. Tkac

2016-05-31 Communication

DOI: 10.1039/C6AN00793G

You might also like

Compound Q&A

What precautions should be taken when handling 2-Methyl-2-propanyl 5-amino-2-thiophenecarboxylate (CAS: 1498311-57-1)?

When handling 2-Methyl-2-propanyl 5-amino-2-thiophenecarboxylate (CAS: 1498311-5...

1498311-57-12-Methyl-2-propanyl ...
Compound Q&A

What are the physical and chemical properties of 5-Bromo-1,2-dichloro-3-fluorobenzene (CAS: 1000572-93-9)?

5-Bromo-1,2-dichloro-3-fluorobenzene (CAS: 1000572-93-9) is a crystalline solid ...

1000572-93-95-Bromo-1,2-dichloro...
Compound Q&A

How should (2R)-2-Amino-2-(4-bromophenyl)ethanol (CAS: 354153-64-3) be stored?

(2R)-2-Amino-2-(4-bromophenyl)ethanol (CAS: 354153-64-3) should be stored in a c...

354153-64-3(2R)-2-Amino-2-(4-br...
Compound Q&A

What regulatory guidelines apply to Methyl 4-(aminomethyl)tetrahydro-2H-pyran-4-carboxylate hydrochloride (CAS: 362707-24-2)?

Methyl 4-(aminomethyl)tetrahydro-2H-pyran-4-carboxylate hydrochloride (CAS: 3627...

362707-24-2Methyl 4-(aminomethy...
Compound Q&A

What are the main uses of 1,4-dimethyl-1H-pyrazole-5-sulfonyl chloride (CAS: 1174834-52-6)?

1,4-Dimethyl-1H-pyrazole-5-sulfonyl chloride is primarily used as an intermediat...

1174834-52-61,4-dimethyl-1H-pyra...
Compound Q&A

Is Dinaphtho[1,2-b:2',1'-d]furan (CAS: 239-69-0) safe?

Dinaphtho[1,2-b:2',1'-d]furan is generally safe when handled with appropriate pe...

239-69-0Dinaphtho[1,2-b:2',1...
Compound Q&A

What is the market or research trend for 7-Methyl-7,9-dihydro-1H-purine-2,6,8(3H)-trione (CAS: 612-37-3)?

The market for 7-Methyl-7,9-dihydro-1H-purine-2,6,8(3H)-trione (CAS: 612-37-3) i...

612-37-37-Methyl-7,9-dihydro...
Compound Q&A

What are the physical and chemical properties of 2-(4-Chlorophenyl)malonaldehyde (CAS: 205676-17-1)?

2-(4-Chlorophenyl)malonaldehyde (CAS: 205676-17-1) is a colorless or light yello...

205676-17-12-(4-Chlorophenyl)ma...
Compound Q&A

How is 2-Methylchrysene (CAS: 3351-32-4) typically synthesized?

2-Methylchrysene (CAS: 3351-32-4) is typically synthesized via the reaction of c...

3351-32-42-Methylchrysene
Compound Q&A

Is N-(6-aminopyrimidin-4-yl)acetamide (CAS: 89533-23-3) safe?

N-(6-aminopyrimidin-4-yl)acetamide (CAS: 89533-23-3) is generally considered saf...

89533-23-3N-(6-aminopyrimidin-...

Source Journal

Polymer Chemistry

Polymer Chemistry
CiteScore: 8.6
Self-citation Rate: 7.3%
Articles per Year: 457

Polymer Chemistry welcomes submissions in all areas of polymer science that have a strong focus on macromolecular chemistry. Manuscripts may cover a broad range of fields, yet no direct application focus is required.

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.