Effect of microwave irradiation on covalent ligand–DNA interactions

Literature Information

Publication Date 2009-04-03
DOI 10.1039/B902357G
Impact Factor 6.222
Authors

Khondaker M. Rahman, David E. Thurston


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Abstract

A 30 second burst of microwave irradiation at an energy level insufficient to cause DNA denaturation or damage drives the covalent reaction between pyrrolobenzodiazepine (PBD) antitumour agents and double-stranded or hairpin oligonucleotides to completion, a process that normally takes between 3–24 hours and thus offering the opportunity for higher-throughput screening of covalent-binding DNA-interactive agents.

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