Effect of microwave irradiation on covalent ligand–DNA interactions
Literature Information
Khondaker M. Rahman, David E. Thurston
A 30 second burst of microwave irradiation at an energy level insufficient to cause DNA denaturation or damage drives the covalent reaction between pyrrolobenzodiazepine (PBD) antitumour agents and double-stranded or hairpin oligonucleotides to completion, a process that normally takes between 3–24 hours and thus offering the opportunity for higher-throughput screening of covalent-binding DNA-interactive agents.
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