Rapid cell mapping using nanoparticles and SERRS
Literature Information
Publication Date
2008-11-19
DOI
10.1039/B815117B
Impact Factor
4.616
Authors
Robert J. Stokes, Fiona McKenzie, Emma McFarlane, Alastair Ricketts, Laurence Tetley, Karen Faulds, James Alexander, Duncan Graham
View Original
Abstract
Bone marrow-derived immune cells (macrophages) treated with gold and silver nanoparticles before fixation and dye staining have been analysed by multiple wavelength line scanning surface enhanced resonance Raman scattering (SERRS) mapping. The method yields high selectivity and sensitivity within short analysis times, identifying nanoparticle aggregates in secondary lysosomes. Using routine cell stains, the output from fluorescence, Raman and SERRS is quantified at four wavelengths of excitation, demonstrating the potential at longer biologically compatible wavelengths of using nanoparticles with cell stains for superior cell mapping.
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Source Journal
Analyst
CiteScore:
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Self-citation Rate:
5.6%
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Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.
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