Synthesis of 5′-amino-5′-deoxyguanosine-5′-N-phosphoramidate and its enzymatic incorporation at the 5′-termini of RNA molecules

Literature Information

Publication Date 2007-10-11
DOI 10.1039/B712066D
Impact Factor 6.222
Authors

David Williamson, Martin J. Cann, David R. W. Hodgson


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Abstract

5′-Amino-5′-deoxyguanosine-5′-N-phosphoramidate (GNHP) was synthesized in four steps from guanosine and was found to initiate T7 RNAP-promoted transcriptions to afford 5′-H2N-RNA that can be conjugated to activated esters.

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