Ultra-smooth glass channels for bioassay with motor proteins
Literature Information
Publication Date
2004-08-09
DOI
10.1039/B409223F
Impact Factor
4.616
Authors
View Original
Abstract
Optically-flat glass channels were fabricated for the non-fluorescent observation of bio-molecules. Two bioassays of motor proteins were successfully performed, in which nanoscale beads and microtubules were clearly observed by a differential interference contrast (DIC) microscope and a dark-field microscope, respectively. The concentration of hydrofluoric acid (HF) was optimized to obtain an optically flat surface which was evaluated by AFM, SEM and dark-field microscopy. A glass channel was etched using a poly(dimethyl siloxane) (PDMS) microfluidic channel as a mask and sealed with a PDMS-coated coverslip. The channel volume of 2–3 µl realized the drastic reduction of proteins required per assay compared with a conventional flow cell method requiring 20 µl.
Related Literature
Hydration of the simplest α-keto acid: a rotational spectroscopic and ab initio study of the pyruvic acid–water complex
Elijah G. Schnitzler, Nathan A. Seifert, Supriya Ghosh, Javix Thomas, Yunjie Xu, Wolfgang Jäger
2017-01-17
Paper
DOI: 10.1039/C6CP08741H
Computational prediction of high thermoelectric performance in p-type half-Heusler compounds with low band effective mass
Teng Fang, Shuqi Zheng, Tian Zhou, Lei Yan, Peng Zhang
2017-01-09
Paper
DOI: 10.1039/C6CP07897D
Anions coordinating anions: analysis of the interaction between anionic Keplerate nanocapsules and their anionic ligands
Josep Bonet Avalos
2017-01-25
Paper
DOI: 10.1039/C6CP08511C
Cyclopentadithiophene organic core in small molecule organic solar cells: morphological control of carrier recombination
Rocío Domínguez, Núria F. Montcada, Pilar de la Cruz, Fernando Langa
2016-12-23
Paper
DOI: 10.1039/C6CP08166E
Corrosion inhibition of copper in aqueous chloride solution by 1H-1,2,3-triazole and 1,2,4-triazole and their combinations: electrochemical, Raman and theoretical studies
Stanley Udochukwu Ofoegbu, Tiago L. P. Galvão, José R. B. Gomes, João Tedim, Helena I. S. Nogueira, M. G. S. Ferreira
2017-02-07
Paper
DOI: 10.1039/C7CP00241F
Mechanism and kinetics of the electrocatalytic reaction responsible for the high cost of hydrogen fuel cells
Tao Cheng, William A Goddard, III, Qi An, Hai Xiao, Boris Merinov, Sergey Morozov
2016-12-21
Communication
DOI: 10.1039/C6CP08055C
Vibrational frequencies and spectroscopic constants of three, stable noble gas molecules: NeCCH+, ArCCH+, and ArCN+
Carlie M. Novak, Ryan C. Fortenberry
2017-01-27
Paper
DOI: 10.1039/C6CP08140A
Molecular interactions of organic molecules at the air/water interface investigated by sum frequency generation vibrational spectroscopy
2017-01-05
Paper
DOI: 10.1039/C6CP07827C
Chemically induced topological zero mode at graphene armchair edges
M. Ziatdinov, H. Lim, S. Fujii, K. Kusakabe, M. Kiguchi, T. Enoki, Y. Kim
2017-01-17
Paper
DOI: 10.1039/C6CP08352H
You might also like
Compound Q&A
What precautions should be taken when handling 4-(2-Furylmethyl)thiomorpholine 1,1-dioxide (CAS: 79206-94-3)?
When handling 4-(2-Furylmethyl)thiomorpholine 1,1-dioxide (CAS: 79206-94-3), it ...
79206-94-34-(2-Furylmethyl)thi...
Compound Q&A
What precautions should be taken when handling 4-Chloro-N-[2-(4-morpholinyl)ethyl]benzamide (CAS: 71320-77-9)?
When handling 4-Chloro-N-[2-(4-morpholinyl)ethyl]benzamide (CAS: 71320-77-9), it...
71320-77-94-Chloro-N-[2-(4-mor...
Compound Q&A
How should waste containing 2-[2-(2-Methoxyethoxy)ethoxy]ethyl 4-methylbenzenesulfonate (CAS: 62921-74-8) be handled?
Waste containing this compound (CAS: 62921-74-8) should be handled according to ...
62921-74-82-[2-(2-Methoxyethox...
Compound Q&A
How should waste containing (S)-Methyl 2-amino-3-cyclohexylpropanoate be handled?
Waste containing (S)-Methyl 2-amino-3-cyclohexylpropanoate should be collected i...
40056-18-6(S)-Methyl 2-amino-3...
Compound Q&A
How is 5-({4-[(2S,4R)-4-Hydroxy-2-methyltetrahydro-2H-pyran-4-yl]-2-thienyl}sulfanyl)-1-methyl-1,3-dihydro-2H-indol-2-one (CAS: 166882-70-8) typically synthesized?
This compound can be synthesized using a multi-step process involving the conjug...
166882-70-85-({4-[(2S,4R)-4-Hyd...
Compound Q&A
Are there alternatives to (2E)-3-(3,4-Dichlorophenyl)acrylic acid (CAS: 7312-27-8) in synthesis?
There are several alternatives to (2E)-3-(3,4-Dichlorophenyl)acrylic acid in syn...
7312-27-8(2E)-3-(3,4-Dichloro...
Compound Q&A
How should Ethyl 6-(2-nitrophenyl)imidazo[2,1-b][1,3]thiazole-3-carboxylate (CAS: 925437-84-9) be stored?
Ethyl 6-(2-nitrophenyl)imidazo[2,1-b][1,3]thiazole-3-carboxylate (CAS: 925437-84...
925437-84-9Ethyl 6-(2-nitrophen...
Compound Q&A
How should waste containing 2-(1,3-Thiazol-2-yl)ethanamine (CAS: 18453-07-1) be handled?
Waste containing 2-(1,3-Thiazol-2-yl)ethanamine (CAS: 18453-07-1) should be coll...
18453-07-12-(1,3-Thiazol-2-yl)...
Compound Q&A
How is Methyl 5-iodo-2-methylbenzoate (CAS: 103440-54-6) typically synthesized?
Methyl 5-iodo-2-methylbenzoate can be synthesized through the iodination of meth...
103440-54-6Methyl 5-iodo-2-meth...
Compound Q&A
How is 5-Chloro[1,2,4]triazolo[1,5-a]pyridine (CAS: 1427399-34-5) typically synthesized?
5-Chloro[1,2,4]triazolo[1,5-a]pyridine is commonly synthesized via the condensat...
1427399-34-55-Chloro[1,2,4]triaz...
Source Journal
Analyst
CiteScore:
7.8
Self-citation Rate:
5.6%
Articles per Year:
653
Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.
Recommended Compounds
(3beta,7beta,12beta)-3,7,12-Trihydroxy-11,15,23-trioxolanost-8-en-26-oic acid
CAS Number:
98665-22-6
Molecular Formula:
C30H44O8
Recommended Suppliers
Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.