Self-assembling monolayer formation of glucose oxidase covalently attached on 11-aminoundecanethiol monolayers on gold

Literature Information

Publication Date 2003-05-29
DOI 10.1039/B303298A
Impact Factor 6.222
Authors

Kenji Doi, Kousuke Tamura, Yosuke Katsumi, Masato Tazaki


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Abstract

Glucose oxidase (GOx) has been attached covalently to form uniform enzyme monolayers on self-assembled monolayers (SAMs) from 11-aminoundecanethiol (AUDT) by taking advantage of chemical oxidation of GOx carbohydrate residues followed by coupling the resulting ‘aldehydic’ enzyme with the terminal amino group in the SAM as characterized by AFM imaging, IR, QCM, and electrochemical measurements.

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