The effect of surface coverage on conformation changes of bovine serum albumin molecules at the air–solution interface detected by sum frequency generation vibrational spectroscopy

Literature Information

Publication Date 2003-04-28
DOI 10.1039/B212551J
Impact Factor 4.616
Authors

Jie Wang, Sarah M. Buck, Zhan Chen


View Original

Abstract

The air–BSA solution interface has been investigated by various techniques for years. From these studies we know that BSA molecules segregate at the BSA solution–air interface, and the surface coverage increases with the increase of the bulk solution concentration. However, questions still remain as to whether the protein changes conformation, orientation, or a combination of the two upon adsorption. In this paper, by using sum frequency generation (SFG) vibrational spectroscopy we found that the conformation of interfacial BSA molecules changes dramatically at the solution–air interface, compared to that of the native BSA in solution. The hydrophobic methyl groups of BSA molecules at this interface tend to align along the surface normal. The degree of such conformational changes of surface BSA molecules depend on the surface coverage, indicating that the protein–protein interaction plays a very important role in determining the conformation of interfacial protein molecules. At very low surface concentration, the adsorbed BSA molecules unfold substantially. Our results can provide a molecular interpretation of results obtained from other studies such as protein layer thickness and surface tension measurements of protein solution.

Related Literature

Nitroreductase-responsive polymeric micelles based on 4-nitrobenzyl and AIE moieties for intracellular doxorubicin release

Xue-Yi Sun, Ya-Xuan Liang, Cheng-Yan Wu, Quan Tang, Rui Liu, Zhong-Lin Lu, Lan He

2021-04-05 Paper

DOI: 10.1039/D1PY00232E

Contents list

2021-04-06 Front/Back Matter

DOI: 10.1039/D1PY90049H

IrAAC-based construction of dual sequence-defined polytriazoles

Xiaojun Wang, Xueyan Zhang, Yong Wang, Shengtao Ding

2021-06-21 Paper

DOI: 10.1039/D1PY00718A

The effect of alkyl chain lengths on the red-to-near-infrared emission of boron-fused azomethine conjugated polymers and their film-state stimuli-responsivities

Shunsuke Ohtani, Natsumi Yamada, Masayuki Gon, Kazuo Tanaka, Yoshiki Chujo

2021-04-19 Paper

DOI: 10.1039/D1PY00213A

Heterobimetallic Au(i)/Y(iii) single chain nanoparticles as recyclable homogenous catalysts

Josina L. Bohlen, Bragavie Kulendran, Hannah Rothfuss, Peter W. Roesky

2021-06-28 Paper

DOI: 10.1039/D1PY00552A

Front cover

2021-04-27 Cover

DOI: 10.1039/D1PY90059E

From 0-dimension to 1-dimensions: Au nanocrystals as versatile plasmonic photocatalyst for broadband light induced RAFT polymerization

Junle Zhang, Mengya Li, Yanjie He, Xiaomeng Zhang, Zhe Cui, Peng Fu, Minying Liu, Xiaoguang Qiao, Qingxiang Zhao, Xinchang Pang

2021-03-25 Paper

DOI: 10.1039/D1PY00088H

Synthetic approaches for copolymers containing nucleic acids and analogues: challenges and opportunities

Hao Lu, Jiansong Cai, Ke Zhang

2021-03-29 Review Article

DOI: 10.1039/D0PY01707H

You might also like

Compound Q&A

How should waste containing 2-Ethyl-4-Methyl-1H-Imidazole-5-Carbaldehyde (CAS: 88634-80-4) be handled?

Waste containing 2-Ethyl-4-Methyl-1H-Imidazole-5-Carbaldehyde (CAS: 88634-80-4) ...

88634-80-42-Ethyl-4-Methyl-1H-...
Compound Q&A

What industries use Triethoxy(octyl)silane (CAS: 1385031-14-0)?

Triethoxy(octyl)silane (CAS: 1385031-14-0) is widely used in the pharmaceuticals...

1385031-14-0Triethoxy(octyl)sila...
Compound Q&A

Are there alternatives to 3-iodo-7-nitro-1H-indazole (CAS: 864724-64-1) in synthesis?

Several alternatives to 3-iodo-7-nitro-1H-indazole (CAS: 864724-64-1) exist in t...

864724-64-13-iodo-7-nitro-1H-in...
Compound Q&A

Are there alternatives to Benzene, bis[(trimethoxysilyl)ethyl] (CAS: 266317-71-9) in synthesis?

Yes, there are alternatives to Benzene, bis[(trimethoxysilyl)ethyl] (CAS: 266317...

266317-71-9Benzene, bis[(trimet...
Compound Q&A

Is Isothiazole-3-carbonitrile (CAS: 1452-17-1) safe?

Isothiazole-3-carbonitrile (CAS: 1452-17-1) is generally considered safe when us...

1452-17-1Isothiazole-3-carbon...
Compound Q&A

Is (3-Chlorophenyl)methanol (CAS: 873-63-2) safe?

(3-Chlorophenyl)methanol (CAS: 873-63-2) is considered low to moderately toxic. ...

873-63-2(3-Chlorophenyl)meth...
Compound Q&A

How is (2S,3S)-2-Hydroxy-3-({[(2-methyl-2-propanyl)oxy]carbonyl}amino)-3-(2-naphthyl)propanoic acid (CAS: 959583-98-3) typically synthesized?

(2S,3S)-2-Hydroxy-3-({[(2-methyl-2-propanyl)oxy]carbonyl}amino)-3-(2-naphthyl)pr...

959583-98-3(2S,3S)-2-Hydroxy-3-...
Compound Q&A

What precautions should be taken when handling Methyl 2-(bromomethyl)-5-methoxybenzoate (CAS: 788081-99-2)?

Proper handling of methyl 2-(bromomethyl)-5-methoxybenzoate requires the use of ...

788081-99-2Methyl 2-(bromomethy...
Compound Q&A

What is 6,8-Dibromoimidazo[1,2-a]pyridine-2-carboxylic acid (CAS: 904805-36-3)?

6,8-Dibromoimidazo[1,2-a]pyridine-2-carboxylic acid (CAS: 904805-36-3) is an aro...

904805-36-36,8-Dibromoimidazo[1...
Compound Q&A

Is 3-Amino-5-bromo-2-pyridinecarbonitrile (CAS: 573675-27-1) safe?

3-Amino-5-bromo-2-pyridinecarbonitrile is considered safe when handled under pro...

573675-27-13-Amino-5-bromo-2-py...

Source Journal

Analyst

Analyst
CiteScore: 7.8
Self-citation Rate: 5.6%
Articles per Year: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.