Detection of a metallo-β-lactamase (IMP-1) by fluorescent probes having dansyl and thiol groups

Literature Information

Publication Date 2002-11-08
DOI 10.1039/B209086D
Impact Factor 3.876
Authors

Hiromasa Kurosaki, Hisami Yasuzawa, Yoshihiro Yamaguchi, Wanchun Jin, Yoshichika Arakawa, Masafumi Goto


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Abstract

A fluorescent probe for the detection of a metallo-β-lactamase (IMP-1), N-[2-(5-dimethylaminonaphthalen-1-ylsulfonylamino)ethyl]-3-mercaptopropionamide (DansylC2SH), 1, was designed based on combining the inhibitory function of mercaptocarboxylate and a fluorophore. The binding of 1 to IMP-1 was investigated by fluorescence spectroscopy. Compound 1 can act as fluorescent probe for detecting IMP-1 selectively.

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